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Wang, T.T.* ; Fekete, A. ; Gáspár, A. ; Ma, J.F.* ; Liang, Z.* ; Yuan, H.M.* ; Zhang, L.H.* ; Schmitt-Kopplin, P. ; Zhang, Y.K.*

Analysis of low molecular weight acids by monolithic immobilized pH gradient-based capillary isoelectric focusing coupled with mass spectrometry.

J. Sep. Sci. 34, 422-427 (2011)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
A novel method for the separation and detection of low molecular weight (LMW) acids was developed using monolithic immobilized pH gradient-based capillary isoelectric focusing coupled with mass spectrometry. Two main parameters, focusing conditions and delivery buffer conditions, which might affect separation efficiency, were optimized with the focusing time of 7 min at 350 V/cm and the delivery buffer of 50% (v/v) acetonitrile in 10 mmol/L ammonium formate (pH 3.0). Under these conditions, the linear correlation between the volume of delivery solvent and the pK(a) of the model components was observed. In addition, the separation mechanism of LMW acids was proposed as well. We suppose that this method may provide a useful tool for the characterization of LMW components (e.g. natural organic matter of different origins).
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Capillary isoelectric focusing; Low molecular weight acids; Mass spectrometry; Monolithic immobilized pH gradient
ISSN (print) / ISBN 1615-9306
e-ISSN 1615-9314
Journal Journal of Separation Science
Quellenangaben Volume: 34, Issue: 4, Pages: 422-427 Article Number: , Supplement: ,
Publisher Wiley
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Ecological Chemistry (IOEC)