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Chen, J.* ; Zhang, X.Y.* ; Cao, R.* ; Lu, X.* ; Zhao, S.M.* ; Fekete, A. ; Huang, Q.* ; Schmitt-Kopplin, P. ; Wang, Y.S.* ; Xu, Z.L.* ; Wan, X.P.* ; Wu, X.H.* ; Zhao, N.Q.* ; Xu, C.J.* ; Xu, G.W.*

Serum 27-nor-5β-Cholestane-3,7,12,24,25 pentol glucuronide discovered by metabolomics as potential diagnostic biomarker for epithelium ovarian cancer.

J. Proteome Res. 10, 2625-2632 (2011)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
The aim of this study was to use a two steps strategy metabolomics to screen/identify and validate novel metabolic biomarker(s) for epithelial ovarian cancer (EOC). In the screening step, serum samples from 27 healthy women, 28 benign ovarian tumors, and 29 EOCs were analyzed by using LC-MS based nontargeted metabolomics. The three groups were separated with OSC filtered PLS-DA model, and six metabolites (27-nor-5β-cholestane-3,7,12,24,25 pentol glucuronide (CPG), phenylalanine, glycocholic acid, propionylcarnitine, Phe-Phe and Lyso PC (18:2)) were considered as potential biomarker candidates. In the validation step, the six metabolites were analyzed in targeted metabolomics by LC-selective ion monitoring mass spectrometry in another 685 serum samples with various clinical backgrounds. As a result, CPG was evaluated to be a potential biomarker and its content was elevated in EOC tissues compared with benign ovarian tumor tissues (p = 0.0005). Besides, CPG levels were found to be up-regulated in early stage EOC and in the three types of EOC histological types. Other variables such as nonovarian diseases, medicine consumption, gynecological inflammations, and menopausal state did not interfere in using CPG as diagnosis marker. CPG was found to be complementary to CA125. Our findings suggest that CPG can be considered a statistical relevant biomarker of EOC, ready for early stage detection.
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Publication type Article: Journal article
Document type Scientific Article
Keywords metabolomics; LC-MS; biomarker discovery; epithelium ovarian cancer; 27-nor-5 beta-cholestane-3,7,12,24,25 pentol glucuronide
Language english
Publication Year 2011
HGF-reported in Year 2011
ISSN (print) / ISBN 1535-3893
e-ISSN 1535-3907
Journal Journal of Proteome Research
Quellenangaben Volume: 10, Issue: 5, Pages: 2625-2632 Article Number: , Supplement: ,
Publisher American Chemical Society (ACS)
Reviewing status Peer reviewed
Institute(s) Institute of Ecological Chemistry (IOEC)
PSP Element(s) G-505190-001
PubMed ID 21456628
DOI 10.1021/pr200173q
Scopus ID 79955799682
Erfassungsdatum 2011-05-23
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