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Nagler, J. ; Schriever, S.C. ; Romanov, A. ; Weisenhorn, D.M. ; Wurst, W. ; Pfluger, P.T. ; Schramm, K.-W.

Determination of morphine and norlaudanosoline in murine brain regions by dispersive liquid-liquid micro-extraction and liquid chromatograpy-electrochemical detection.

Neurochem. Int. 150:105174 (2021)
Postprint DOI PMC
Open Access Green
Morphine can be synthesized endogenously by mammals from dopamine via the intermediate norlaudanosoline. Previously, both compounds have been detected separately in whole brains of mice and brain regions of rats, and in urine of humans. Here, we report a novel method for the analysis of both compounds in single murine brain regions. Initially, a variant of dispersive liquid-liquid microextraction was established by using methanol as an extractant, cyclohexane as solvent, and tributylphosphate as disperser. The extraction method was applied to murine brain regions homogenized with perchloric acid while the subsequent detection was carried out by HPLC with electrochemical detection. In the thalamus of C57Bl/6J mice (n = 3, male, age 4-8 months), morphine and norlaudanosoline could be detected at levels of 19 ± 3.9 and 7.2 ± 2.3 pg/mg, respectively. Overall, we provide a novel method for the simultaneous extraction and detection of both morphine and norlaudanosoline in single murine brain regions.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Dispersive Liquid-liquid Microextraction ; Electrochemical Detection ; Liquid Chromatography ; Morphine ; Murine Brain Regions ; Norlaudanosoline ; Parkinson's Disease; Endogenous Morphine; Tetrahydropapaveroline; Microextraction; Metabolites; Preconcentration; Localization; Dopamine
ISSN (print) / ISBN 0197-0186
Journal Neurochemistry International
Quellenangaben Volume: 150, Issue: , Pages: , Article Number: 105174 Supplement: ,
Publisher Elsevier
Publishing Place Amsterdam
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) PPM-MEX-Molecular EXposomics (MEX)
Institute of Diabetes and Obesity (IDO)
Institute of Developmental Genetics (IDG)
Grants DFG