PuSH - Publication Server of Helmholtz Zentrum München

Sato, K.* ; Mascher, M.* ; Himmelbach, A.* ; Haberer, G. ; Spannagl, M. ; Stein, N.*

Chromosome-scale assembly of wild barley accession "OUH602".

Genes Genomes Genetics G3 11:jkab244 (2021)
Publ. Version/Full Text Research data DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Barley (Hordeum vulgare) was domesticated from its wild ancestral form ca. 10,000 years ago in the Fertile Crescent and is widely cultivated throughout the world, except for in tropical areas. The genome size of both cultivated barley and its conspecific wild ancestor is approximately 5 Gb. High-quality chromosome-level assemblies of 19 cultivated and one wild barley genotype were recently established by pan-genome analysis. Here, we release another equivalent short-read assembly of the wild barley accession "OUH602." A series of genetic and genomic resources were developed for this genotype in prior studies. Our assembly contains more than 4.4 Gb of sequence, with a scaffold N50 value of over 10 Mb. The haplotype shows high collinearity with the most recently updated barley reference genome, "Morex" V3, with some inversions. Gene projections based on "Morex" gene models revealed 46,807 protein-coding sequences and 43,375 protein-coding genes. Alignments to publicly available sequences of bacterial artificial chromosome (BAC) clones of "OUH602" confirm the high accuracy of the assembly. Since more loci of interest have been identified in "OUH602," the release of this assembly, with detailed genomic information, should accelerate gene identification and the utilization of this key wild barley accession.
Altmetric
Additional Metrics?
[➜Log in]
Edit extra informations Login
Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Hordeum Vulgare Ssp. Spontaneum ; Ouh602 ; Genome Assembly ; Pseudomolecules ; Wild Barley; Quantitative Trait Loci; Multiple Disease Resistance
ISSN (print) / ISBN 2160-1836
e-ISSN 2160-1836
Journal Genes Genomes Genetics G3
Quellenangaben Volume: 11, Issue: 10, Pages: , Article Number: jkab244 Supplement: ,
Publisher Genetics Society of America
Publishing Place Pittsburgh, PA
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Research Unit Plant Genome and Systems Biology (PGSB)
Grants German Ministry of Education and Research project SHAPE I
German Ministry of Education and Research project de.NBI
JST Mirai Program grant Japan