PuSH - Publication Server of Helmholtz Zentrum München: Simultaneous calcium imaging and glucose stimulation in living zebrafish to investigate in vivo β-cell function.

Navigation

Home

Deutsch

Research

Advanced Search

Browse by ...

... Journal

... Publication Type

... Research Data

... Publication Year

Publication overview

Support & Contact

Contact persons

Help

Data protection

Delgadillo-Silva, L.F.* ; Akhtar, M.N.* ; Taşöz, E.* ; Ninov, N.

Simultaneous calcium imaging and glucose stimulation in living zebrafish to investigate in vivo β-cell function.

J. Vis. Exp. 2021:e62347 (2021)

DOI

PMC

Open Access Green as soon as Postprint is submitted to ZB.

Abstract
Metrics
Extra information

The pancreatic β-cells sustain systemic glucose homeostasis by producing and secreting insulin according to the blood glucose levels. Defects in β-cell function are associated with hyperglycemia that can lead to diabetes. During the process of insulin secretion, β-cells experience an influx of Ca²⁺. Thus, imaging the glucose-stimulated Ca²⁺ influx using genetically encoded calcium indicators (GECIs) provides an avenue to studying β-cell function. Previously, studies showed that isolated zebrafish islets expressing GCaMP6s exhibit significant Ca²⁺ activity upon stimulation with defined glucose concentrations. However, it is paramount to study how β-cells respond to glucose not in isolation, but in their native environment, where they are systemically connected, vascularized, and densely innervated. To this end, the study leveraged the optical transparency of the zebrafish larvae at early stages of development to illuminate β-cell activity in vivo. Here, a detailed protocol for Ca²⁺ imaging and glucose stimulation to investigate β-cell function in vivo is presented. This technique allows to monitor the coordinated Ca²⁺ dynamics in β-cells with single-cell resolution. Additionally, this method can be applied to work with any injectable solution such as small molecules or peptides. Altogether, the protocol illustrates the potential of the zebrafish model to investigate islet coordination in vivo and to characterize how environmental and genetic components might affect β-cell function.

Altmetric

Additional Metrics?

[➜Log in]

Edit extra informations Login

Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Ca2+ Dynamics; Islets

ISSN (print) / ISBN 1940-087X

e-ISSN 1940-087X

Journal Journal of Visualized Experiments

Quellenangaben Volume: 2021, Issue: 175, Article Number: e62347

Publisher JoVE

Publishing Place 1 Alewife Center, Ste 200, Cambridge, Ma 02140 Usa

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Institute for Pancreatic Beta Cell Research (IPI)

Grants Immunological and Cellular Strategies in Metabolic Disease
International Research Training Group
German Research Foundation (DFG)
German Center for Diabetes Research (DZD)
Center for Regenerative Therapies Dresden at TU Dresden