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Kalla, D.* ; Flisikowski, K.* ; Yang, K. ; Sangüesa, L.B.* ; Kurome, M.* ; Kessler, B.* ; Zakhartchenko, V.* ; Wolf, E.* ; Lickert, H. ; Saur, D.* ; Schnieke, A.* ; Flisikowska, T.*

The missing link: Cre pigs for cancer research.

Front. Oncol. 11:755746 (2021)
Publ. Version/Full Text Research data DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
The Cre/loxP system is a powerful tool for the generation of animal models with precise spatial and temporal gene expression. It has proven indispensable in the generation of cancer models with tissue specific expression of oncogenes or the inactivation of tumor suppressor genes. Consequently, Cre-transgenic mice have become an essential prerequisite in basic cancer research. While it is unlikely that pigs will ever replace mice in basic research they are already providing powerful complementary resources for translational studies. But, although conditionally targeted onco-pigs have been generated, no Cre-driver lines exist for any of the major human cancers. To model human pancreatic cancer in pigs, Cre-driver lines were generated by CRISPR/Cas9-mediated insertion of codon-improved Cre (iCre) into the porcine PTF1A gene, thus guaranteeing tissue and cell type specific function which was proven using dual fluorescent reporter pigs. The method used can easily be adapted for the generation of other porcine Cre-driver lines, providing a missing tool for modeling human cancers in large animals.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Cell Fate ; Genome Editing ; Icre Recombinase ; Pancreatic Cancer ; Pig ; Ptf1a ; Translational Model; Ptf1a; Recombinase; Expression; Promoter; Models
Language english
Publication Year 2021
HGF-reported in Year 2021
ISSN (print) / ISBN 2234-943X
e-ISSN 2234-943X
Journal Frontiers in Oncology
Quellenangaben Volume: 11, Issue: , Pages: , Article Number: 755746 Supplement: ,
Publisher Frontiers
Publishing Place Avenue Du Tribunal Federal 34, Lausanne, Ch-1015, Switzerland
Reviewing status Peer reviewed
Institute(s) Institute of Diabetes and Regeneration Research (IDR)
POF-Topic(s) 90000 - German Center for Diabetes Research
30201 - Metabolic Health
Research field(s) Helmholtz Diabetes Center
PSP Element(s) G-501900-231
G-502300-001
DOI 10.3389/fonc.2021.755746
WOS ID WOS:000710950700001
Scopus ID 85117560561
PubMed ID 34692545
Erfassungsdatum 2021-12-09
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