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Pan, M.* ; Kohlbauer, V.* ; Blancke Soares, A.* ; Schinke, H.* ; Huang, Y.* ; Kranz, G.* ; Quadt, T.* ; Hachmeister, M.* ; Gires, O.

Interactome analysis reveals endocytosis and membrane recycling of EpCAM during differentiation of embryonic stem cells and carcinoma cells.

iScience 24:103179 (2021)
Publ. Version/Full Text Research data DOI PMC
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Transmembrane epithelial cell adhesion molecule (EpCAM) is expressed in epithelia, carcinoma, teratoma, and embryonic stem cells (ESCs). EpCAM displays spatiotemporal patterning during embryogenesis, tissue morphogenesis, cell differentiation, and epithelial-to-mesenchymal transition (EMT) in carcinomas. Potential interactors of EpCAM were identified in murine F9 teratoma cells using a stable isotope labeling with amino acids in cell culture-based proteomic approach (n = 77, enrichment factor >3, p value ≤ 0.05). Kyoto Encyclopedia of Genes and Genomes and gene ontology terms revealed interactions with regulators of endosomal trafficking and membrane recycling, which were further validated for Rab5, Rab7, and Rab11. Endocytosis and membrane recycling of EpCAM were confirmed in mF9 cells, E14TG2α ESC, and Kyse30 carcinoma cells. Reduction of EpCAM during mesodermal differentiation and TGFβ-induced EMT correlated with enhanced endocytosis and block or reduction of recycling in ESCs and esophageal carcinoma cells. Hence, endocytosis and membrane recycling are means of regulation of EpCAM protein levels during differentiation of ESC and EMT induction in carcinoma cells.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Cancer ; Cell Biology ; Stem Cells Research; Adhesion Molecule Epcam; Breast-cancer; Tight Junction; Ep-cam; Expression; Antigen; Tumor; Cleavage; Acidification; Contributes
Language english
Publication Year 2021
HGF-reported in Year 2021
ISSN (print) / ISBN 2589-0042
e-ISSN 2589-0042
Journal iScience
Quellenangaben Volume: 24, Issue: 10, Pages: , Article Number: 103179 Supplement: ,
Publisher Elsevier
Publishing Place Amsterdam ; Bosten ; London ; New York ; Oxford ; Paris ; Philadelphia ; San Diego ; St. Louis
Reviewing status Peer reviewed
Institute(s) Translational Metabolic Oncology (IDC-TMO)
POF-Topic(s) 30504 - Mechanisms of Genetic and Environmental Influences on Health and Disease
Research field(s) Radiation Sciences
PSP Element(s) G-521800-001
Grants German Research Council (Deutsche Forschungsgemeinschaft)
DOI 10.1016/j.isci.2021.103179
WOS ID WOS:000713816400003
Scopus ID 85122746238
PubMed ID 34693227
Erfassungsdatum 2021-12-16
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