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Ivorra, L.* ; Cruzeiro, C. ; Ramos, A.* ; Tagulao, K.* ; Cardoso, P.G.*

How can environmental conditions influence dicofol genotoxicity on the edible Asiatic clam, Meretrix meretrix?

Environ. Pollut. 293:118467 (2022)

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Open Access Green as soon as Postprint is submitted to ZB.

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Genotoxic effects of dicofol on the edible clam Meretrix meretrix were investigated through a mesocosm experiment. Individuals of M. meretrix, were exposed to environmental concentration (D1 = 50 ng/L) and supra-environmental concentration (D2 = 500 ng/L) of dicofol for 15 days, followed by the same depuration period. DNA damage (i.e., strand breaks and alkali-labile sites) was evaluated at day 1, 7 and 15, during uptake and depuration, using Comet assay (alkaline version) and nuclear abnormalities (NAs) as genotoxicity biomarkers. The protective effects of dicofol against DNA damage induced by ex vivo hydrogen peroxide (H₂O₂) exposure were also assessed. Comet assay results revealed no significant DNA damages under dicofol exposure, indicating 1) apparent lack of genotoxicity of dicofol to the tested conditions and/or 2) resistance of the animals due to optimal adaptation to stress conditions. Moreover, ex vivo H₂O₂ exposure showed an increase in the DNA damage in all the treatments without significant differences between them. However, considering only the DNA damage induced by H₂O₂ during uptake phase, D1 animals had significantly lower DNA damage than those from other treatments, revealing higher protection against a second stressor. NAs data showed a decrease in the % of cells with polymorphic, kidney shape, notched or lobbed nucleus, along the experiment. The combination of these results supports the idea that the clams used in the experiment were probably collected from a stressful environment (in this case Pearl River Delta region) which could have triggered some degree of adaptation to those environmental conditions, explaining the lack of DNA damages and highlighting the importance of organisms' origin and the conditions that they were exposed during their lives.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Bivalves ; Comet Assay ; Dna Damage ; Nuclear Abnormalities ; Organochlorine Pesticides; Mussels Perna-viridis; Dna Strand Breakage; Mytilus-galloprovincialis; Organochlorine Pesticides; Nuclear Abnormalities; Damage; Hemocytes; Comet; Exposure; Erythrocytes

ISSN (print) / ISBN 0269-7491

e-ISSN 1873-6424

Journal Environmental Pollution

Quellenangaben Volume: 293, Article Number: 118467

Publisher Elsevier

Publishing Place The Boulevard, Langford Lane, Kidlington, Oxford Ox5 1gb, Oxon, England

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Research Unit Comparative Microbiome Analysis (COMI)

Grants Portuguese Science and Technology Foundation (FCT)
Macao Science and Technology Development Fund