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Tarquis Medina, M. ; Scheibner, K. ; Gonzales García, I. ; Bastidas-Ponce, A. ; Sterr, M. ; Jaki, J. ; Schirge, S. ; García-Cáceres, C. ; Lickert, H. ; Bakhti, M.

Synaptotagmin-13 is a neuroendocrine marker in brain, intestine and pancreas.

Int. J. Mol. Sci. 22:12526 (2021)
Publ. Version/Full Text Research data DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Synaptotagmin-13 (Syt13) is an atypical member of the vesicle trafficking synaptotagmin protein family. The expression pattern and the biological function of this Ca2+-independent protein are not well resolved. Here, we have generated a novel Syt13-Venus fusion (Syt13-VF) fluorescence reporter allele to track and isolate tissues and cells expressing Syt13 protein. The reporter allele is regulated by endogenous cis-regulatory elements of Syt13 and the fusion protein follows an identical expression pattern of the endogenous Syt13 protein. The homozygous reporter mice are viable and fertile. We identify the expression of the Syt13-VF reporter in different regions of the brain with high expression in tyrosine hydroxylase (TH)-expressing and oxytocin-producing neuroendocrine cells. Moreover, Syt13-VF is highly restricted to all enteroendocrine cells in the adult intestine that can be traced in live imaging. Finally, Syt13-VF protein is expressed in the pancreatic endocrine lineage, allowing their specific isolation by flow sorting. These findings demonstrate high expression levels of Syt13 in the endocrine lineages in three major organs harboring these secretory cells. Collectively, the Syt13-VF reporter mouse line provides a unique and reliable tool to dissect the spatio-temporal expression pattern of Syt13 and enables isolation of Syt13-expressing cells that will aid in deciphering the molecular functions of this protein in the neuroendocrine system.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Brain ; Endocrine Lineage ; Fluorescent Reporter ; Intestine ; Pancreas ; Synaptotagmin-13 ; Syt13; Messenger-rna; Expression; Ca2+; Family
ISSN (print) / ISBN 1422-0067
e-ISSN 1661-6596
Journal International Journal of Molecular Sciences
Quellenangaben Volume: 22, Issue: 22, Pages: , Article Number: 12526 Supplement: ,
Publisher MDPI
Publishing Place Basel
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Diabetes and Regeneration Research (IDR)
Institute of Diabetes and Obesity (IDO)
Grants Deutsches Zentrum fur Diabetesforschung (DZD)
Helmholtz Zentrum Munchen