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Candeias, J. ; Schmidt-Weber, C.B. ; Buters, J.T.M.

Dosing intact birch pollen grains at the airliquid interface (ALI) to the immortalized human bronchial epithelial cell line BEAS-2B.

PLoS ONE 16:e0259914 (2021)

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In real life, humans are exposed to whole pollen grains at the air epithelial barrier. We developed a system for in vitro dosing of whole pollen grains at the Air-Liquid Interface (ALI) and studied their effect on the immortalized human bronchial epithelial cell line BEAS-2B. Pollen are sticky and large particles. Dosing pollen needs resuspension of single particles rather than clusters, and subsequent transportation to the cells with little loss to the walls of the instrumentation i.e. in a straight line. To avoid high speed impacting insults to cells we chose sedimentation by gravity as a delivery step. Pollen was resuspended into single particles by pressured air. A pollen dispersion unit including PTFE coating of the walls and reduced air pressure limited impaction loss to the walls. The loss of pollen to the system was still about 40%. A linear dose effect curve resulted in 327-2834 pollen/cm2 (± 6.1%), the latter concentration being calculated as the amount deposited on epithelial cells on high pollen days. After whole pollen exposure, the largest differential gene expression at the transcriptomic level was late, about 7 hours after exposure. Inflammatory and response to stimulus related genes were up-regulated. We developed a whole pollen exposure air-liquid interface system (Pollen-ALI), in which cells can be gently and reliably dosed.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Bet V 1; Adhesion Molecule-1 Icam-1; Grass-pollen; Allergen Exposure; Dendritic Cells; Kappa-b; Challenge; Chamber; Responses; Release

ISSN (print) / ISBN 1932-6203

Journal PLoS ONE

Quellenangaben Volume: 16, Issue: 11, Article Number: e0259914

Publisher Public Library of Science (PLoS)

Publishing Place Lawrence, Kan.

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Institute for Allergy Research (IAF)

Grants Helmholtz Center Munich, Munich, Germany in the frame of Task Force Allergy, subject "Air"