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Westhoff Smith, D.* ; Chakravorty, A.* ; Hayes, M.* ; Hammerschmidt, W. ; Sugden, B.*

The Epstein-Barr virus oncogene EBNA1 suppresses natural killer cell responses and apoptosis early after infection of peripheral B cells.

mBio 12:e0224321 (2021)
Publ. Version/Full Text DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
The innate immune system serves as frontline defense against pathogens, such as bacteria and viruses. Natural killer (NK) cells are a part of innate immunity and can both secrete cytokines and directly target cells for lysis. NK cells express several cell surface receptors, including NKG2D, which bind multiple ligands. People with deficiencies in NK cells are often susceptible to uncontrolled infection by herpesviruses, such as Epstein-Barr virus (EBV). Infection with EBV stimulates both innate and adaptive immunity, yet the virus establishes lifelong latent infection in memory B cells. We show that the EBV oncogene EBNA1, previously known to be necessary for maintaining EBV genomes in latently infected cells, also plays an important role in suppressing NK cell responses and cell death in newly infected cells. EBNA1 does so by downregulating the NKG2D ligands ULBP1 and ULBP5 and modulating expression of c-Myc. B cells infected with a derivative of EBV that lacks EBNA1 are more susceptible to NK cell-mediated killing and show increased levels of apoptosis. Thus, EBNA1 performs a previously unappreciated role in reducing immune response and programmed cell death after EBV infection, helping infected cells avoid immune surveillance and apoptosis and thus persist for the lifetime of the host. IMPORTANCE Epstein-Barr virus (EBV) is a ubiquitous human pathogen, infecting up to 95% of the world's adult population. Initial infection with EBV can cause infectious mononucleosis. EBV is also linked to several human malignancies, including lymphomas and carcinomas. Although infection by EBV alerts the immune system and causes an immune response, the virus persists for life in memory B cells. We show that the EBV protein EBNA1 can downregulate several components of the innate immune system linked to natural killer (NK) cells. This downregulation of NK cell activity translates to lower killing of EBV-infected cells and is likely one way that EBV escapes immune surveillance after infection. Additionally, we show that EBNA1 reduces apoptosis in newly infected B cells, allowing more of these cells to survive. Taken together, our findings uncover new functions of EBNA1 and provide insights into viral strategies to survive the initial immune response postinfection.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords B Cell ; Epstein-barr Virus ; Ulbp ; C-myc ; Herpesviruses ; Innate Immunity ; Natural Killer Cells; Antigen 1 Ebna1; Nuclear Antigen; Dna-damage; Gene-expression; H2ax Phosphorylation; Latent Infection; Plasmid Origin; Nk Cells; T-cells; Nkg2d
ISSN (print) / ISBN 2150-7511
e-ISSN 2150-7511
Journal mBio
Quellenangaben Volume: 12, Issue: 6, Pages: , Article Number: e0224321 Supplement: ,
Publisher American Society for Microbiology (ASM)
Publishing Place 1752 N St Nw, Washington, Dc 20036-2904 Usa
Non-patent literature Publications
Reviewing status Peer reviewed
Grants NCI NIH HHS