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Horsch, M. ; Seeburg, PH.* ; Adler, T.* ; Aguilar-Pimentel, J.A. ; Becker, L.* ; Calzada-Wack, J.* ; Garrett, L. ; Götz, A. ; Hans, W. ; Higuchi, M.* ; Hölter, S.M. ; Naton, B. ; Prehn, C. ; Puk, O. ; Rácz, I.* ; Rathkolb, B.* ; Rozman, J.* ; Schrewe, A. ; Adamski, J. ; Busch, D.H.* ; Esposito, I.* ; Graw, J. ; Ivandic, B.* ; Klingenspor, M.* ; Klopstock, T.* ; Mempel, M. ; Ollert, M.* ; Schulz, S. ; Wolf, E.* ; Wurst, W. ; Zimmer, A.* ; Gailus-Durner, V. ; Fuchs, H. ; Hrabě de Angelis, M. ; Beckers, J.

Requirement of the RNA-editing enzyme ADAR2 for normal physiology in mice.

J. Biol. Chem. 286, 18614-18622 (2011)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
ADAR2, an RNA editing enzyme that converts specific adenosines to inosines in certain pre-mRNAs, often leading to amino acid substitutions in the encoded proteins, is mainly expressed in brain. Of all ADAR2-mediated edits, a single one in the pre-mRNA of the AMPA receptor subunit GluA2 is essential for survival. Hence, early postnatal death of mice lacking ADAR2 is averted when the critical edit is engineered into both GluA2 encoding Gria2 alleles. Adar2(-/-)/Gria2(R/R) mice display normal appearance and life span, but the general phenotypic effects of global lack of ADAR2 have remained unexplored. Here we have employed the Adar2(-/-)/Gria2(R/R) mouse line, and Gria2(R/R) mice as controls, to study the phenotypic consequences of loss of all ADAR2-mediated edits except the critical one in GluA2. Our extended phenotypic analysis covering ∼320 parameters identified significant changes related to absence of ADAR2 in behavior, hearing ability, allergy parameters and transcript profiles of brain.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords no keywords
ISSN (print) / ISBN 0021-9258
e-ISSN 1083-351X
Journal Journal of Biological Chemistry, The
Quellenangaben Volume: 286, Issue: 21, Pages: 18614-18622 Article Number: , Supplement: ,
Publisher American Society for Biochemistry and Molecular Biology
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Experimental Genetics (IEG)
Molekulare Endokrinologie und Metabolismus (MEM)
Institute of Developmental Genetics (IDG)
Institute of Pathology (PATH)
Institute of Epidemiology (EPI)
CCG Environmental Dermatology and Allergology (ILBD-KAU)
Institute of Epidemiology (EPI)
Research Unit Molecular Epidemiology (AME)