PuSH - Publication Server of Helmholtz Zentrum München

Qarri, A. ; Rinkevich, Y. ; Rinkevich, B.*

Employing marine invertebrate cell culture media for isolation and cultivation of thraustochytrids.

Bot. Marina 64, 447-454 (2021)
DOI
Thraustochytrids, a common group of marine eukaryotic protists, have drawn considerable scientific and industrial interest due to their ability to synthesize high levels of bioactive compounds, including polyunsaturated fatty acids, docosahexaenoic acid, squalene and carotenoids, and their new applications for biofuels. The pharmaceutical and industrial potential of thraustochytrids necessitate effective isolation of new strains and establishment of axenic cultures. To date, existing isolation protocols have used baiting and direct plating methods to generate axenic cultures with varied media compositions that contain peptone and yeast extracts as nitrogen sources, glucose as carbon source, seawater and antibiotics. Here we reveal a new approach for the isolation of thraustochytrids from tissues of marine invertebrates using (a) primary cell cultures in a liquid medium containing basal medium, 50% artificial seawater, vitamins, proteins and antibiotics, and (b) cultivation in the same cell culture medium. Using the colonial tunicate Botryllus schlosseri as a model system, thraustochytrid cells thrived in the medium from the day of extraction, grew and proliferated for the next five weeks (five-passages, up to 1.9 x 10(6) cells ml(-1) in passage 5; 1.45-fold multiplication week(-1)). This new approach for isolation and cultivation of axenic thraustochytrid cultures enables the isolation of new species with promising bioactive compounds.
Altmetric
Additional Metrics?
[➜Log in]
Edit extra informations Login
Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Ascidians ; Cultivation ; Labyrinthulomycetes ; Marine Invertebrates ; Pufa; Polyunsaturated Fatty-acids; Botryllus-schlosseri; Establishment; Strains; Protists; Fungi; Lines
ISSN (print) / ISBN 0006-8055
e-ISSN 1437-4323
Journal Botanica Marina
Quellenangaben Volume: 64, Issue: 6, Pages: 447-454 Article Number: , Supplement: ,
Publisher de Gruyter
Publishing Place Genthiner Strasse 13, D-10785 Berlin, Germany
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Regenerative Biology and Medicine (IRBM)
Institute of Lung Health and Immunity (LHI)
Grants European Research Council
Else-Kroner-Fresenius-Stiftung
Fritz-Thyssen-Stiftung
German Research Foundation
Human Frontier Science Program Career Development Award