Home
Deutsch
Advanced Search
Browse by ...
Publication overview
Contact persons
Help
Publ. Version/Full Text
DOI
PMC
 |
Open Access Gold |
|
as soon as is submitted to ZB.
Differentiation of human pluripotent stem cells into pancreatic duct-like organoids.
STAR Protoc. 2:100913 (2021)
The recapitulation of human developmental processes and pathological manifestations requires access to specific cell types and precursor stages during embryogenesis and disease. Here, we describe a scalable in vitro differentiation protocol to guide human pluripotent stem cells stepwise into pancreatic duct-like organoids. The protocol mimics pancreatic duct development and was successfully used to model the onset and progression of pancreatic ductal adenocarcinoma; the approach is suitable for multiple downstream applications. However, the protocol is cost- and time-intensive. For complete details on the use and execution of this protocol, please refer to Breunig et al. (2021).
Altmetric
Additional Metrics?
Edit extra informations
Login
Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Cell Culture ; Cell Differentiation ; Developmental Biology ; Organoids ; Stem Cells
e-ISSN
2666-1667
Journal
STAR Protocols
Quellenangaben
Volume: 2,
Issue: 4,
Article Number: 100913
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Helmholtz Pioneer Campus (HPC)
Grants
Deutsche Krebshilfe (Germany)
Else Kroner-Forschungskolleg (Else Kroner-Fresenius-Stiftung) of the Technical University of Munich (TUM)
'Deutsche Forschungsgemeinschaft (DFG)'
Else Kroner-Forschungskolleg (Else Kroner-Fresenius-Stiftung) of the Technical University of Munich (TUM)
'Deutsche Forschungsgemeinschaft (DFG)'