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Amar, Y.* ; Schneider, E. ; Köberle, M.* ; Seeholzer, T. ; Musiol, S. ; Hölge, I.M.* ; Gschwendtner, S. ; Krappmann, D. ; Steiger, K.* ; Biedermann, T.* ; Schmidt-Weber, C.B. ; Alessandrini, F.

Microbial dysbiosis in a mouse model of atopic dermatitis mimics shifts in human microbiome and correlates with the key pro-inflammatory cytokines IL-4, IL-33 and TSLP.

J. Eur. Acad. Dermatol. Venereol. 36, 705-716 (2022)

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BACKGROUND: Cutaneous bacterial dysbiosis is a characteristic hallmark of atopic dermatitis (AD) and it decisively influences the severity of the disease. Despite this, frequently used murine models of AD have not been characterized regarding the changes in skin microbiome communities. OBJECTIVE: To analyze the skin microbiome of two frequently used murine models for AD for assessing their applicability in translational research. METHODS: AD was induced in mice by topical application of calcipotriol, or oxazolone. Following comparable elicitation of AD-like dermatitis, including IgE induction, the skin microbial communities were analyzed and compared with human AD. RESULTS: We detected critical differences in the microbiota composition of diseased skin. In contrast to calcipotriol treatment, application of oxazolone induced significant changes of the cutaneous microbiota and a drastic drop of bacterial richness. Furthermore, an expansion of Staphylococci, particularly S. xylosus was observed in the oxazolone group, also displaying positive correlations with AD key markers including pH, TEWL, IL-4, TSLP and IL-33. CONCLUSIONS: In this article we show that i) the model of choice to investigate AD needs to be characterized for the cutaneous microbiota if applicable and ii) the oxazolone-mediated mixed Th1-Th2 immune response triggers microbiota-induced alterations which share similarities to dysbiosis in human AD and represents therefore a suitable model for translational research on AD if alterations of the microbiome are in the focus of the investigation.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Microbiome ; Atopic Dermatitis ; Mouse Models; Thymic Stromal Lymphopoietin; Skin Microbiome; Stratum-corneum; Antimicrobial Peptides; Barrier Disruption; Water-loss; Expression; Mice; Keratinocytes; Ph

ISSN (print) / ISBN 0926-9959

e-ISSN 1468-3083

Journal Journal of the European Academy of Dermatology and Venereology

Quellenangaben Volume: 36, Issue: 5, Pages: 705-716

Publisher Wiley

Publishing Place 111 River St, Hoboken 07030-5774, Nj Usa

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Institute for Allergy Research (IAF)
Research Unit Signaling and Translation (SAT)
Research Unit Comparative Microbiome Analysis (COMI)

Grants Deutsche Forschungsgemeinschaft
Federal Ministry of Food and Agriculture (BMEL)