PuSH - Publication Server of Helmholtz Zentrum München: An adipose lncRAP2-Igf2bp2 complex enhances adipogenesis and energy expenditure by stabilizing target mRNAs.

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Alvarez-Dominguez, J.R.* ; Winther, S.* ; Hansen, J.B.* ; Lodish, H.F.* ; Knoll, M.

An adipose lncRAP2-Igf2bp2 complex enhances adipogenesis and energy expenditure by stabilizing target mRNAs.

iScience 25:103680 (2022)

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	Open Access Gold

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lncRAP2 is a conserved cytoplasmic lncRNA enriched in adipose tissue and required for adipogenesis. Using purification and in vivo interactome analyses, we show that lncRAP2 forms complexes with proteins that stabilize mRNAs and modulate translation, among them Igf2bp2. Surveying transcriptome-wide Igf2bp2 client mRNAs in white adipocytes reveals selective binding to mRNAs encoding adipogenic regulators and energy expenditure effectors, including adiponectin. These same target proteins are downregulated when either Igf2bp2 or lncRAP2 is downregulated, hindering adipocyte lipolysis. Proteomics and ribosome profiling show this occurs predominantly through mRNA accumulation, as lncRAP2-Igf2bp2 complex binding does not impact translation efficiency. Phenome-wide association studies reveal specific associations of genetic variants within both lncRAP2 and Igf2bp2 with body mass and type 2 diabetes, and both lncRAP2 and Igf2bp2 are suppressed in adipose depots of obese and diabetic individuals. Thus, the lncRAP2-Igf2bp2 complex potentiates adipose development and energy expenditure and is associated with susceptibility to obesity-linked diabetes.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Biological Sciences ; Molecular Biology ; Molecular Physiology ; Omics; Long Noncoding Rnas; Genome-wide Association; Body-mass Index; Binding-protein; Gene-expression; Tissue; Translation; Regulators; Loci; Discovery

ISSN (print) / ISBN 2589-0042

e-ISSN 2589-0042

Journal iScience

Quellenangaben Volume: 25, Issue: 1, Article Number: 103680

Publisher Elsevier

Publishing Place Amsterdam ; Bosten ; London ; New York ; Oxford ; Paris ; Philadelphia ; San Diego ; St. Louis

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Institute of Diabetes Research Type 1 (IDF)

Grants National Institute of Health
Deutsche Forschungsgemeinschaft