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Chaves-Arquero, B.* ; Martinez Lumbreras, S. ; Camero, S.* ; Santiveri, C.M.* ; Mirassou, Y.* ; Campos-Olivas, R.* ; Jimenez, M.* ; Calvo, O.* ; Pérez-Cañadillas, J.M.*

Structural basis of Nrd1-Nab3 heterodimerization.

Life Sci. All. 5:e202101252 (2022)

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Heterodimerization of RNA binding proteins Nrd1 and Nab3 is essential to communicate the RNA recognition in the nascent transcript with the Nrd1 recognition of the Ser₅-phosphorylated Rbp1 C-terminal domain in RNA polymerase II. The structure of a Nrd1-Nab3 chimera reveals the basis of heterodimerization, filling a missing gap in knowledge of this system. The free form of the Nrd1 interaction domain of Nab3 (NRID) forms a multi-state three-helix bundle that is clamped in a single conformation upon complex formation with the Nab3 interaction domain of Nrd1 (NAID). The latter domain forms two long helices that wrap around NRID, resulting in an extensive protein-protein interface that would explain the highly favorable free energy of heterodimerization. Mutagenesis of some conserved hydrophobic residues involved in the heterodimerization leads to temperature-sensitive phenotypes, revealing the importance of this interaction in yeast cell fitness. The Nrd1-Nab3 structure resembles the previously reported Rna14/Rna15 heterodimer structure, which is part of the poly(A)-dependent termination pathway, suggesting that both machineries use similar structural solutions despite they share little sequence homology and are potentially evolutionary divergent.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Rna-polymerase-ii; Transcription Termination; Binding Proteins; Molecular-basis; Recognition; Ctd; Phosphorylation; Reveals; Snorna

ISSN (print) / ISBN 2575-1077

e-ISSN 2575-1077

Journal Life Science Alliance

Quellenangaben Volume: 5, Issue: 4, Article Number: e202101252

Publisher EMBO Press

Publishing Place Heidelberg

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Institute of Structural Biology (STB)

Grants "Junta de Castilla y Leon" (Programa "Escalera de Excelencia" de la Junta de Castilla y Leon - O.P. ERDF from Castilla y Leon 14-20)
ESFESF
ERDF
Biomedicine program of Community of Madrid
ERDF A way of making Europe
MCIN/AEI