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Belda, E.* ; Voland, L.* ; Tremaroli, V.* ; Falony, G.* ; Adriouch, S.* ; Assmann, K.E.* ; Prifiti, E.* ; Aron-Wisnewsky, J.* ; Debédat, J.* ; Le Roy, T.* ; Nielsen, T.* ; Amouyal, C.* ; Andre, S.* ; Andreelli, F.* ; Blüher, M.* ; Chakaroun, R.* ; Chilloux, J.* ; Coelho, L.P.* ; Dao, M.C.* ; Das, P.* ; Fellahi, S.* ; Forslund, S.K.* ; Galleron, N.* ; Hansen, T.H.* ; Holmes, B.* ; Ji, B.* ; Krogh Pedersen, H.* ; Le, P.* ; Le Chatelier, E.* ; Lewinter, C.* ; Mannerås-Holm, L.* ; Marquet, F.* ; Myridakis, A.* ; Pelloux, V.* ; Pons, N.* ; Quinquis, B.* ; Rouault, C.* ; Roume, H.* ; Salem, J.E.* ; Sokolovska, N.* ; Søndertoft, N.B.* ; Touch, S.* ; Vieira-Silva, S.* ; Galan, P.* ; Holst, J.* ; Gøtze, J.P.* ; Køber, L.* ; Vestergaard, H.* ; Hansen, T.* ; Hercberg, S.* ; Oppert, J.M.* ; Nielsen, J.* ; Letunic, I.* ; Dumas, M.E.* ; Stumvoll, M. ; Pedersen, O.B.* ; Bork, P.* ; Ehrlich, S.D.* ; Zucker, J.D.* ; Bäckhed, F.* ; Raes, J.* ; Clément, K.*

Impairment of gut microbial biotin metabolism and host biotin status in severe obesity: Effect of biotin and prebiotic supplementation on improved metabolism.

Gut 71, 2463-2480 (2022)
Postprint Research data DOI PMC
Open Access Gold (Paid Option)
Creative Commons Lizenzvertrag
OBJECTIVES: Gut microbiota is a key component in obesity and type 2 diabetes, yet mechanisms and metabolites central to this interaction remain unclear. We examined the human gut microbiome's functional composition in healthy metabolic state and the most severe states of obesity and type 2 diabetes within the MetaCardis cohort. We focused on the role of B vitamins and B7/B8 biotin for regulation of host metabolic state, as these vitamins influence both microbial function and host metabolism and inflammation. DESIGN: We performed metagenomic analyses in 1545 subjects from the MetaCardis cohorts and different murine experiments, including germ-free and antibiotic treated animals, faecal microbiota transfer, bariatric surgery and supplementation with biotin and prebiotics in mice. RESULTS: Severe obesity is associated with an absolute deficiency in bacterial biotin producers and transporters, whose abundances correlate with host metabolic and inflammatory phenotypes. We found suboptimal circulating biotin levels in severe obesity and altered expression of biotin-associated genes in human adipose tissue. In mice, the absence or depletion of gut microbiota by antibiotics confirmed the microbial contribution to host biotin levels. Bariatric surgery, which improves metabolism and inflammation, associates with increased bacterial biotin producers and improved host systemic biotin in humans and mice. Finally, supplementing high-fat diet-fed mice with fructo-oligosaccharides and biotin improves not only the microbiome diversity, but also the potential of bacterial production of biotin and B vitamins, while limiting weight gain and glycaemic deterioration. CONCLUSION: Strategies combining biotin and prebiotic supplementation could help prevent the deterioration of metabolic states in severe obesity. TRIAL REGISTRATION NUMBER: NCT02059538.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Diabetes Mellitus ; Intestinal Bacteria ; Micronutrients ; Nutrition ; Obesity; Chromium Picolinate; Expression; Diet; Overweight; Health
ISSN (print) / ISBN 0017-5749
e-ISSN 1468-3288
Journal Gut (eGut)
Quellenangaben Volume: 71, Issue: 12, Pages: 2463-2480 Article Number: , Supplement: ,
Publisher BMJ Publishing Group
Publishing Place British Med Assoc House, Tavistock Square, London Wc1h 9jr, England
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Helmholtz Institute for Metabolism, Obesity and Vascular Research (HI-MAG)
Grants JPI-Microdiet study
French Agency of Research (ANR-CAPTOR, ANR-DeepIntegromics)
Fondation pour la Recherche Medicale
Deutsche Forschungsgemeinschaft (DFG)
LeDucq Foundation
European Union