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Duffner, C. ; Kublik, S. ; Fösel, B. ; Frostegård,* ; Schloter, M. ; Bakken, L.* ; Schulz, S.

Genotypic and phenotypic characterization of hydrogenotrophic denitrifiers.

Environ. Microbiol. 24, 1887-1901 (2022)
Publ. Version/Full Text Research data DOI PMC
Open Access Gold (Paid Option)
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Stimulating litho-autotrophic denitrification in aquifers with hydrogen is a promising strategy to remove excess NO3 - , but it often entails accumulation of the cytotoxic intermediate NO2 - and the greenhouse gas N2 O. To explore if these high NO2 - and N2 O concentrations are caused by differences in the genomic composition, the regulation of gene transcription or the kinetics of the reductases involved, we isolated hydrogenotrophic denitrifiers from a polluted aquifer, performed whole-genome sequencing and investigated their phenotypes. We therefore assessed the kinetics of NO2 - , NO, N2 O, N2 and O2 as they depleted O2 and transitioned to denitrification with NO3 - as the only electron acceptor and hydrogen as the electron donor. Isolates with a complete denitrification pathway, although differing intermediate accumulation, were closely related to Dechloromonas denitrificans, Ferribacterium limneticum or Hydrogenophaga taeniospiralis. High NO2 - accumulation was associated with the reductases' kinetics. While available, electrons only flowed towards NO3 - in the narG-containing H. taeniospiralis but flowed concurrently to all denitrification intermediates in the napA-containing D. denitrificans and F. limneticum. The denitrification regulator RegAB, present in the napA strains, may further secure low intermediate accumulation. High N2 O accumulation only occurred during the transition to denitrification and is thus likely caused by delayed N2 O reductase expression.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Sulfate-reducing Bacteria; Decompose Fatty-acids; Autohydrogenotrophic Denitrification; Gen-nov; Nitrate; Water; Biofilm; Groundwater; Kinetics; Growth
ISSN (print) / ISBN 1462-2912
e-ISSN 1462-2920
Journal Environmental Microbiology
Quellenangaben Volume: 24, Issue: 4, Pages: 1887-1901 Article Number: , Supplement: ,
Publisher Wiley
Publishing Place 111 River St, Hoboken 07030-5774, Nj Usa
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Research Unit Comparative Microbiome Analysis (COMI)
Grants Deutsche Forschungsgemeinschaft