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The priming effect of diesel exhaust on native pollen exposure at the air-liquid interface.
Environ. Res. 211:112968 (2022)
Publ. Version/Full Text
Research data
DOI
PMC
Pollen related allergic diseases have been increasing for decades. The reasons for this increase are unknown, but environmental pollution like diesel exhaust seem to play a role. While previous studies explored the effects of pollen extracts, we studied here for the first time priming effects of diesel exhaust on native pollen exposure using a novel experimental setup. Methods: Human bronchial epithelial BEAS-2B cells were exposed to native birch pollen (real life intact pollen, not pollen extracts) at the air-liquid interface (pollen-ALI). BEAS-2B cells were also pre-exposed in a diesel-ALI to diesel CAST for 2 h (a model for diesel exhaust) and then to pollen in the pollen-ALI 24 h later. Effects were analysed by genome wide transcriptome analysis after 2 h 25 min, 6 h 50 min and 24 h. Selected genes were confirmed by qRT-PCR. Results: Bronchial epithelial cells exposed to native pollen showed the highest transcriptomic changes after about 24 h. About 3157 genes were significantly up- or down-regulated for all time points combined. After pre-exposure to diesel exhaust the maximum reaction to pollen had shifted to about 2.5 h after exposure, plus the reaction to pollen was desensitised as only 560 genes were differentially regulated. Only 97 genes were affected synergistically. Of these, enrichment analysis showed that genes involved in immune and inflammatory response were involved. Conclusion: Diesel exhaust seems to prime cells to react more rapidly to native pollen exposure, especially inflammation related genes, a factor known to facilitate the development of allergic sensitization. The marker genes here detected could guide studies in humans when investigating whether modern and outdoor diesel exhaust exposure is still detrimental for the development of allergic disease.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Air-liquid Interface ; Beas-2b ; Fresh Diesel Model Exhaust ; Native Pollen ; Pollen Chamber
ISSN (print) / ISBN
0013-9351
e-ISSN
1096-0953
Journal
Environmental Research
Quellenangaben
Volume: 211,
Article Number: 112968
Publisher
Elsevier
Publishing Place
San Diego, Calif.
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute for Allergy Research (IAF)
Cooperation Group Comprehensive Molecular Analytics (CMA)
Cooperation Group Comprehensive Molecular Analytics (CMA)
Grants
Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschun
Helmholtz Center Munich , Munich, Germany in the frame of Task Force Allergy
Helmholtz Center Munich , Munich, Germany in the frame of Task Force Allergy