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Acetogens and acetoclastic methanosarcinales govern methane formation in abandoned coal mines.
Appl. Environ. Microbiol. 77, 3749-3756 (2011)
Publ. Version/Full Text Volltext
DOI
PMC
In abandoned coal mines, methanogenic archaea are responsible for the production of substantial amounts of methane. The present study aimed to directly unravel the active methanogens mediating methane release as well as active bacteria potentially involved in the trophic network. Therefore, the stable-isotope-labeled precursors of methane, [(13)C]acetate and H(2)-(13)CO(2), were fed to liquid cultures from hard coal and mine timber from a coal mine in Germany. Guided by methane production rates, samples for DNA stable-isotope probing (SIP) with subsequent quantitative PCR and denaturing gradient gel electrophoretic (DGGE) analyses were taken over 6 months. Surprisingly, the formation of [(13)C]methane was linked to acetoclastic methanogenesis in both the [(13)C]acetate- and the H(2)-(13)CO(2)-amended cultures of coal and timber. H(2)-(13)CO(2) was used mainly by acetogens related to Pelobacter acetylenicus and Clostridium species. Active methanogens, closely affiliated with Methanosarcina barkeri, utilized the readily available acetate rather than the thermodynamically more favorable hydrogen. Thus, the methanogenic microbial community appears to be highly adapted to the low-H(2) conditions found in coal mines.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
powder river-basin; 16s ribosomal-rna; microbial communities; illinois basin; gas; bacteria; usa; bioremediation; methanogenesis; populations
ISSN (print) / ISBN
0099-2240
e-ISSN
1098-5336
Quellenangaben
Volume: 77,
Issue: 11,
Pages: 3749-3756
Publisher
American Society for Microbiology (ASM)
Publishing Place
Washington, USA
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Groundwater Ecology (IGOE)