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A protocol for CRISPR-mediated activation and repression of human endogenous retroviruses in human pluripotent stem cells.
STAR Protoc. 3:101281 (2022)
Human endogenous retroviruses (HERVs) comprise many regulatory elements and can regulate host gene activity at different expression levels via multiple mechanisms. Here, we introduce a step-by-step protocol to activate or repress transcription of HERV-K(HML-2) elements using the CRISPRa and CRISPRi technologies in human embryonic stem cells. This protocol can help deciphering the functional role of HERV-K(HML-2) elements in critical biological processes. The protocol may easily be adapted to other cell lines and HERV groups with relatively low sequence heterogeneity. For complete details on the use and execution of this protocol, please refer to Padmanabhan Nair et al. (2021).
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Crispr ; Developmental Biology
e-ISSN
2666-1667
Journal
STAR Protocols
Quellenangaben
Volume: 3,
Issue: 2,
Article Number: 101281
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Virology (VIRO)
Grants
Deutsche Forschungsgemeinschaft
Medical Faculty of University of Saarland
Medical Faculty of University of Saarland