PuSH - Publication Server of Helmholtz Zentrum München: Development and validation of a 6-gene signature for the prognosis of loco-regional control in patients with HPV-negative locally advanced HNSCC treated by postoperative radio(chemo)therapy.

Navigation

Home

Deutsch

Research

Advanced Search

Browse by ...

... Journal

... Publication Type

... Research Data

... Publication Year

Publication overview

Support & Contact

Contact persons

Help

Data protection

Patil, S.* ; Linge, A.* ; Grosser, M.* ; Lohaus, F.* ; Gudziol, V.* ; Kemper, M.* ; Nowak, A.* ; Haim, D.* ; Tinhofer, I.* ; Budach, V.* ; Guberina, M.* ; Stuschke, M.* ; Balermpas, P.* ; Rödel, C.* ; Schäfer, H.* ; Grosu, A.L.* ; Abdollahi, A.* ; Debus, J.* ; Ganswindt, U.* ; Belka, C. ; Pigorsch, S.* ; Combs, S.E. ; Boeke, S.* ; Zips, D.* ; Baretton, G.B.* ; Baumann, M.* ; Krause, M.* ; Löck, S.*

Development and validation of a 6-gene signature for the prognosis of loco-regional control in patients with HPV-negative locally advanced HNSCC treated by postoperative radio(chemo)therapy.

Radiother. Oncol. 171, 91-100 (2022)

DOI

PMC

Open Access Green as soon as Postprint is submitted to ZB.

Abstract
Metrics
Extra information

PURPOSE: The aim of this study was to develop and validate a novel gene signature from full-transcriptome data using machine-learning approaches to predict loco-regional control (LRC) of patients with human papilloma virus (HPV)-negative locally advanced head and neck squamous cell carcinoma (HNSCC), who received postoperative radio(chemo)therapy (PORT-C). MATERIALS AND METHODS: Gene expression analysis was performed using Affymetrix GeneChip Human Transcriptome Array 2.0 on a multicentre retrospective training cohort of 128 patients and an independent validation cohort of 114 patients from the German Cancer Consortium - Radiation Oncology Group (DKTK-ROG). Genes were filtered based on differential gene expression analyses and Cox regression. The identified gene signature was combined with clinical parameters and with previously identified genes related to stem cells and hypoxia. Technical validation was performed using nanoString technology. RESULTS: We identified a 6-gene signature consisting of four individual genes CAV1, GPX8, IGLV3-25, TGFBI, and one metagene combining the highly correlated genes INHBA and SERPINE1. This signature was prognostic for LRC on the training data (ci=0.84) and in validation (ci=0.63) with a significant patient stratification into two risk groups (p=0.005). Combining the 6-gene signature with the clinical parameters T stage and tumour localisation as well as the stem-cell marker CD44 and the 15-gene hypoxia-associated signature improved the validation performance (ci=0.69, p=0.001). CONCLUSION: We have developed and validated a novel prognostic 6-gene signature for LRC of HNSCC patients with HPV-negative tumours treated by PORT-C. After successful prospective validation the signature can be part of clinical trials on the individualization of radiotherapy.

Altmetric

Additional Metrics?

[➜Log in]

Edit extra informations Login

Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Cancer Stem Cells ; Gene Signature ; Head And Neck Squamous Cell Carcinoma ; Hypoxia ; Machine Learning ; Postoperative Radiotherapy

ISSN (print) / ISBN 0167-8140

e-ISSN 1879-0887

Journal Radiotherapy and Oncology

Quellenangaben Volume: 171, Pages: 91-100

Publisher Elsevier

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) CCG Personalized Radiotherapy in Head and Neck Cancer (KKG-KRT)
Institute of Radiation Medicine (IRM)

Grants Nationales Centrum für Tumorerkrankungen Heidelberg
Universidad Católica de Córdoba
TNTB
Normal and Tumor Tissue Bank
National Center for Tumor Diseases