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Sachs, C. ; Kanaparthi, D. ; Kublik, S. ; Szalay, A.A. ; Schloter, M. ; Damgaard, L.R.* ; Schramm, A.* ; Lueders, T.*

Tracing long-distance electron transfer and cable bacteria in freshwater sediments by agar pillar gradient columns.

FEMS Microbiol. Ecol. 98:fiac042 (2022)

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Cable bacteria (CB) perform electrogenic sulphur oxidation (e-SOX) by spatially separating redox-half-reactions over cm-distances. For freshwater systems, the ecology of CB is not yet well understood, partly because they proved difficult to cultivate. This study introduces a new "agar pillar" approach to selectively enrich and investigate CB-populations. Within sediment columns, a central agar pillar is embedded, providing a sediment-free gradient-system in equilibrium with the surrounding sediment. We incubated freshwater sediments from a streambed, a sulfidic lake, and a hydrocarbon polluted aquifer in such agar pillar columns. Microprofiling revealed typical patterns of e-SOx, such as the development of a suboxic zone and the establishment of electric potentials. The bacterial communities in the sediments and agar pillars were analysed over depth by PacBio near-full-length 16S rRNA gene amplicon sequencing, allowing for a precise phylogenetic placement of taxa detected. The selective niche of the agar pillar was preferentially colonized by CB related to Candidatus Electronema for surface-water sediments, including several potentially novel species, but not for putative groundwater CB affiliated with Desulfurivibrio spp. The presence of CB was seemingly linked to co-enriched fermenters, hinting at a possible role of e-SOx-populations as an electron sink for heterotrophic microbes. These findings add to our current understanding of the diversity and ecology of CB in freshwater systems, and to a discrimination of CB from surface and groundwater sediments. The agar pillar approach provides a new strategy that may facilitate the cultivation of redox gradient-dependent microorganisms, including previously unrecognized CB populations.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Pacbio Near-full-length 16s Rrna Gene Amplicon Sequencing ; Agar Pillar Columns ; Electrogenic Sulphur Oxidation (e-sox) ; Freshwater Cable Bacteria ; Sediment Microprofiling

ISSN (print) / ISBN 0168-6496

e-ISSN 1574-6941

Journal FEMS Microbiology Ecology

Quellenangaben Volume: 98, Issue: 5, Article Number: fiac042

Publisher Wiley

Publishing Place Oxford

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Institute of Groundwater Ecology (IGOE)
Research Unit Comparative Microbiome Analysis (COMI)

Grants European Research Council