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Analysis of innate immune responses against pathogenic bacteria in Arabidopsis, tomato, and barley.
Methods Mol. Biol. 2494, 269-289 (2022)
The immune status of plants can be evaluated by monitoring the propagation of pathogens. Plants defend themselves against pathogen attack through an intricate network of phytohormone-driven innate immune responses. Of these, salicylic acid (SA)-dependent defense responses can be assessed in planta by monitoring the propagation of biotrophic and hemi-biotrophic pathogens. Here, we describe methods to monitor the propagation of the hemi-biotrophic bacterial pathogen Pseudomonas syringae in Arabidopsis thaliana leaves. We describe protocols to (i) propagate the plants to the appropriate growth stage for infection, (ii) prepare the bacterial inoculum, (iii) inoculate plants using spray and infiltration techniques, and (iv) analyze the resulting in planta bacterial titers. The latter bacterial titers serve as a measure of plant susceptibility and negatively correlate with immunity. Based on the methods used with the A. thaliana-P. syringae model pathosystem, we include complementary methods allowing the analysis of innate immunity in the crop plants Solanum lycopersicum (tomato) in interaction with P. syringae and Hordeum vulgare (barley) in interaction with Xanthomonas translucens.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Arabidopsis Thaliana ; Bacterial Titer Determination ; Barley ; Infiltration ; Pathogen Infection ; Plant Immunity ; Pseudomonas Syringae ; Spray Inoculation ; Tomato ; Xanthomonas Translucens
ISSN (print) / ISBN
1064-3745
e-ISSN
1940-6029
Book Volume Title
Environmental Responses in Plants
Journal
Methods in Molecular Biology
Quellenangaben
Volume: 2494,
Pages: 269-289
Publisher
Springer
Publishing Place
Berlin [u.a.]
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Biochemical Plant Pathology (BIOP)