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Boutegrabet, L. ; Fekete, A. ; Hertkorn, N. ; Papastamoulis, Y.* ; Waffo-Téguo, P.* ; Mérillon, J.M.* ; Jeandet, P.* ; Gougeon, R.D.* ; Schmitt-Kopplin, P.

Determination of stilbene derivatives in Burgundy red wines by ultra-high-pressure liquid chromatography.

Anal. Bioanal. Chem. 401, 1513-1521 (2011)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
The polyphenols, for example stilbenes and flavonoids, are an important family of compounds present in grapes and wines. Several studies have shown that stilbenes are antioxidants and cancer-preventing agents. For the first time, eight natural stilbenes (trans-resveratrol, trans-piceid, cis-piceid, trans-astringin, trans-piceatannol, (+)-trans-ε-viniferin, pallidol, and hopeaphenol), isolated and purified from Vitis vinifera, were simultaneously analysed by ultra-high-pressure liquid chromatography coupled with photodiode-array detection. Separation of the stilbenes by UHPLC was optimized with the assistance of "Quality-by-Design" commercial software. Four different reversed-phase columns packed with 1.5-1.7-μm particles were tested and compared for their retention behaviour and separation efficiency. On the basis of the performance characteristics determined, the VisionHT C18 HL column was selected for the stilbenes studied, because resolution of the critical pair was 1.5 with a peak width of 2-4 s. The optimized method resulted in highly repeatable retention times (RSD 0.03-0.07%), peak areas (RSD 3-6%), and linear ranges were between 0.005 and 50 mg L-1 for most of the compounds. All stilbenes, except trans-astringin, trans-piceatannol, and pallidol were identified and quantified in Burgundy red wines at different concentrations after direct injection of the wines. [Figure not available: see fulltext.] © 2011 Springer-Verlag.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Chromatographic Columns ; Quality-by-design ; Stilbenes ; Uhplc ; Wine
ISSN (print) / ISBN 1618-2642
e-ISSN 1618-2650
Journal Analytical and Bioanalytical Chemistry
Quellenangaben Volume: 401, Issue: 5, Pages: 1513-1521 Article Number: , Supplement: ,
Publisher Springer
Publishing Place Heidelberg
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Ecological Chemistry (IOEC)