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Grisard, E.C.* ; Nevo, N.* ; Lescure, A.* ; Doll, S. ; Corbé, M.* ; Jouve, M.* ; Lavieu, G.* ; Joliot, A.* ; Nery, E.D.* ; Martin-Jaular, L.* ; Théry, C.*

Homosalate boosts the release of tumour-derived extracellular vesicles with protection against anchorage-loss property.

J. Extra. Vesicles 11:e12242 (2022)

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Eukaryotic cells, including cancer cells, secrete highly heterogeneous populations of extracellular vesicles (EVs). EVs could have different subcellular origin, composition and functional properties, but tools to distinguish between EV subtypes are scarce. Here, we tagged CD63- or CD9-positive EVs secreted by triple negative breast cancer cells with Nanoluciferase enzyme, to set-up a miniaturized method to quantify secretion of these two EV subtypes directly in the supernatant of cells. We performed a cell-based high-content screening to identify clinically-approved drugs able to affect EV secretion. One of the identified hits is Homosalate, an anti-inflammatory drug found in sunscreens which robustly increased EVs' release. Comparing EVs induced by Homosalate with those induced by Bafilomycin A1, we demonstrate that: (1) the two drugs act on EVs generated in distinct subcellular compartments, and (2) EVs released by Homosalate-, but not by Bafilomycin A1-treated cells enhance resistance to anchorage loss in another recipient epithelial tumour cell line. In conclusion, we identified a new drug modifying EV release and demonstrated that under influence of different drugs, triple negative breast cancer cells release EV subpopulations from different subcellular origins harbouring distinct functional properties.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

ISSN (print) / ISBN 2001-3078

e-ISSN 2001-3078

Journal Journal of extracellular vesicles

Quellenangaben Volume: 11, Issue: 7, Article Number: e12242

Publisher Taylor & Francis

Publishing Place [S.l.]

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Institute of Metabolism and Cell Death (MCD)