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Niu, Z. ; Prade, E.* ; Malideli, E.* ; Hille, K.* ; Jussupow, A.* ; Mideksa, Y.G.* ; Yan, L.M.* ; Qian, C.* ; Fleisch, M. ; Messias, A.C. ; Sarkar, R. ; Sattler, M. ; Lamb, D.C.* ; Feige, M.J.* ; Camilloni, C.* ; Kapurniotu, A.* ; Reif, B.

Structural insight into IAPP-derived amyloid inhibitors and their mechanism of action.

Adv. Mater. 59, 5820-5830 (2020)
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Open Access Hybrid
Creative Commons Lizenzvertrag
Designed peptides derived from the islet amyloid polypeptide (IAPP) cross-amyloid interaction surface with Aβ (termed interaction surface mimics or ISMs) have been shown to be highly potent inhibitors of Aβ amyloid self-assembly. However, the molecular mechanism of their function is not well understood. Using solution-state and solid-state NMR spectroscopy in combination with ensemble-averaged dynamics simulations and other biophysical methods including TEM, fluorescence spectroscopy and microscopy, and DLS, we characterize ISM structural preferences and interactions. We find that the ISM peptide R3-GI is highly dynamic, can adopt a β-like structure, and oligomerizes into colloid-like assemblies in a process that is reminiscent of liquid–liquid phase separation (LLPS). Our results suggest that such assemblies yield multivalent surfaces for interactions with Aβ40. Sequestration of substrates into these colloid-like structures provides a mechanistic basis for ISM function and the design of novel potent anti-amyloid molecules.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Amyloid-bildung ; Amyloid-inhibitoren ; Aβ ; Festkörper-nmr-spektroskopie ; Peptide
Language english
Publication Year 2020
HGF-reported in Year 2020
ISSN (print) / ISBN 0935-9648
e-ISSN 1521-4095
Journal Advanced materials
Quellenangaben Volume: 59, Issue: 14, Pages: 5820-5830 Article Number: , Supplement: ,
Publisher Wiley
Publishing Place Weinheim
Reviewing status Peer reviewed
Institute(s) Institute of Structural Biology (STB)
POF-Topic(s) 30203 - Molecular Targets and Therapies
Research field(s) Enabling and Novel Technologies
PSP Element(s) G-503090-001
G-503000-001
DOI 10.1002/ANGE.201914559
Scopus ID 85134876354
Erfassungsdatum 2022-09-02
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