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van den Hoek, H. ; Klena, N.* ; Jordan, M.A.* ; Viar, G.A.* ; Righetto, R.D. ; Schaffer, M.* ; Erdmann, P.S.* ; Wan, W.* ; Geimer, S.* ; Plitzko, J.M.* ; Baumeister, W.* ; Pigino, G.* ; Hamel, V.* ; Guichard, P.* ; Engel, B.D.

In situ architecture of the ciliary base reveals the stepwise assembly of intraflagellar transport trains.

Science 377, 543-548 (2022)
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The cilium is an antenna-like organelle that performs numerous cellular functions, including motility, sensing, and signaling. The base of the cilium contains a selective barrier that regulates the entry of large intraflagellar transport (IFT) trains, which carry cargo proteins required for ciliary assembly and maintenance. However, the native architecture of the ciliary base and the process of IFT train assembly remain unresolved. In this work, we used in situ cryo-electron tomography to reveal native structures of the transition zone region and assembling IFT trains at the ciliary base in Chlamydomonas. We combined this direct cellular visualization with ultrastructure expansion microscopy to describe the front-to-back stepwise assembly of IFT trains: IFT-B forms the backbone, onto which bind IFT-A, dynein-1b, and finally kinesin-2 before entry into the cilium
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Publication type Article: Journal article
Document type Scientific Article
Language english
Publication Year 2022
HGF-reported in Year 2022
ISSN (print) / ISBN 0036-8075
e-ISSN 1095-9203
Journal Science
Quellenangaben Volume: 377, Issue: 6605, Pages: 543-548 Article Number: , Supplement: ,
Publisher American Association for the Advancement of Science (AAAS)
Reviewing status Peer reviewed
Institute(s) Helmholtz Pioneer Campus (HPC)
POF-Topic(s) 30203 - Molecular Targets and Therapies
Research field(s) Pioneer Campus
PSP Element(s) G-510008-001
DOI 10.1126/science.abm6704
WOS ID WOS:000836647500044
Scopus ID 85135161846
PubMed ID 35901159
Erfassungsdatum 2022-11-07
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