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Hofstatter, P.G.* ; Thangavel, G.* ; Lux, T. ; Neumann, P.* ; Vondrak, T.* ; Novak, P.* ; Zhang, M.* ; Costa, L.T.* ; Castellani, M.* ; Scott, A.* ; Toegelová, H.* ; Fuchs, J.* ; Mata-Sucre, Y.* ; Dias, Y.* ; Vanzela, A.L.L.* ; Huettel, B.* ; Almeida, C.C.S.* ; Šimková, H.* ; Souza, G.* ; Pedrosa-Harand, A.* ; Macas, J.* ; Mayer, K.F.X. ; Houben, A.* ; Marques, A.R.*

Repeat-based holocentromeres influence genome architecture and karyotype evolution.

Cell 185, 3153-3168.e18 (2022)
Publ. Version/Full Text Research data DOI PMC
Open Access Gold (Paid Option)
Creative Commons Lizenzvertrag
The centromere represents a single region in most eukaryotic chromosomes. However, several plant and animal lineages assemble holocentromeres along the entire chromosome length. Here, we compare genome organization and evolution as a function of centromere type by assembling chromosome-scale holocentric genomes with repeat-based holocentromeres from three beak-sedge (Rhynchospora pubera, R. breviuscula, and R. tenuis) and their closest monocentric relative, Juncus effusus. We demonstrate that transition to holocentricity affected 3D genome architecture by redefining genomic compartments, while distributing centromere function to thousands of repeat-based centromere units genome-wide. We uncover a complex genome organization in R. pubera that hides its unexpected octoploidy and describe a marked reduction in chromosome number for R. tenuis, which has only two chromosomes. We show that chromosome fusions, facilitated by repeat-based holocentromeres, promoted karyotype evolution and diploidization. Our study thus sheds light on several important aspects of genome architecture and evolution influenced by centromere organization.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Centromere ; Dysploidy ; Genome Regulation ; Holocentric Chromosomes ; Rhynchospora ; Spatial Genome Organization ; Transposable Elements ; Whole-genome Duplication
ISSN (print) / ISBN 0092-8674
e-ISSN 1097-4172
Journal Cell
Quellenangaben Volume: 185, Issue: 17, Pages: 3153-3168.e18 Article Number: , Supplement: ,
Publisher Cell Press
Publishing Place Cambridge, Mass.
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Research Unit Plant Genome and Systems Biology (PGSB)
Grants Max-Planck-Gesellschaft
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
Deutscher Akademischer Austauschdienst
Elixir CZ Research Infrastructure Project
DAAD/India