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Koch, F.* ; Fenouil, R.* ; Gut, M.* ; Cauchy, P.* ; Albert, T.K.* ; Zacarias-Cabeza, J.* ; Spicuglia, S.* ; de la Chapelle, A.L.* ; Heidemann, M. ; Hintermair, C. ; Eick, D. ; Gut, I.* ; Ferrier, P.* ; Andrau, J.C.*

Transcription initiation platforms and GTF recruitment at tissue-specific enhancers and promoters.

Nat. Struct. Mol. Biol. 18, 956-963 (2011)
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Recent work has shown that RNA polymerase (Pol) II can be recruited to and transcribe distal regulatory regions. Here we analyzed transcription initiation and elongation through genome-wide localization of Pol II, general transcription factors (GTFs) and active chromatin in developing T cells. We show that Pol II and GTFs are recruited to known T cell-specific enhancers. We extend this observation to many new putative enhancers, a majority of which can be transcribed with or without polyadenylation. Importantly, we also identify genomic features called transcriptional initiation platforms (TIPs) that are characterized by large areas of Pol II and GTF recruitment at promoters, intergenic and intragenic regions. TIPs show variable widths (0.4-10 kb) and correlate with high CpG content and increased tissue specificity at promoters. Finally, we also report differential recruitment of TFIID and other GTFs at promoters and enhancers. Overall, we propose that TIPs represent important new regulatory hallmarks of the genome.
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Publication type Article: Journal article
Document type Scientific Article
Keywords RNA-polymerase-II; Cell-specific enhancer; Genome-wide analysis; Gene-expression; T-Cells; Regulatory elements; Locus; Activation; Chromatin; Identification
Language english
Publication Year 2011
HGF-reported in Year 2011
ISSN (print) / ISBN 1545-9993
e-ISSN 1545-9985
Journal Nature Structural & Molecular Biology
Quellenangaben Volume: 18, Issue: 8, Pages: 956-963 Article Number: , Supplement: ,
Publisher Nature Publishing Group
Publishing Place New York, NY
Reviewing status Peer reviewed
Institute(s) Institute of Clinical Molecular Biology and Tumor Genetics (K.MOLBI)
POF-Topic(s) 30504 - Mechanisms of Genetic and Environmental Influences on Health and Disease
Research field(s) Immune Response and Infection
PSP Element(s) G-501490-001
PubMed ID 21765417
DOI 10.1038/nsmb.2085
Scopus ID 79961022267
Erfassungsdatum 2011-10-14
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