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McLellan, H.* ; Harvey, S.E.* ; Steinbrenner, J.* ; Armstrong, M.R.* ; He, Q.* ; Clewes, R.* ; Pritchard, L.* ; Wang, W.* ; Wang, S.* ; Nussbaumer, T. ; Dohai, B.S.M. ; Luo, Q.* ; Kumari, P.* ; Duan, H.* ; Roberts, A.* ; Boevink, P.C.* ; Neumann, C.* ; Champouret, N.* ; Hein, I.* ; Falter-Braun, P. ; Beynon, J.* ; Denby, K.* ; Birch, P.R.J.*

Exploiting breakdown in nonhost effector-target interactions to boost host disease resistance.

Proc. Natl. Acad. Sci. U.S.A. 119:e2114064119 (2022)
Postprint Research data DOI PMC
Open Access Hybrid
Creative Commons Lizenzvertrag
Plants are resistant to most microbial species due to nonhost resistance (NHR), providing broad-spectrum and durable immunity. However, the molecular components contributing to NHR are poorly characterised. We address the question of whether failure of pathogen effectors to manipulate nonhost plants plays a critical role in NHR. RxLR (Arg-any amino acid-Leu-Arg) effectors from two oomycete pathogens, Phytophthora infestans and Hyaloperonospora arabidopsidis, enhanced pathogen infection when expressed in host plants (Nicotiana benthamiana and Arabidopsis, respectively) but the same effectors performed poorly in distantly related nonhost pathosystems. Putative target proteins in the host plant potato were identified for 64 P. infestans RxLR effectors using yeast 2-hybrid (Y2H) screens. Candidate orthologues of these target proteins in the distantly related non-host plant Arabidopsis were identified and screened using matrix Y2H for interaction with RxLR effectors from both P. infestans and H. arabidopsidis. Few P. infestans effector-target protein interactions were conserved from potato to candidate Arabidopsis target orthologues (cAtOrths). However, there was an enrichment of H. arabidopsidis RxLR effectors interacting with cAtOrths. We expressed the cAtOrth AtPUB33, which unlike its potato orthologue did not interact with P. infestans effector PiSFI3, in potato and Nicotiana benthamiana. Expression of AtPUB33 significantly reduced P. infestans colonization in both host plants. Our results provide evidence that failure of pathogen effectors to interact with and/or correctly manipulate target proteins in distantly related non-host plants contributes to NHR. Moreover, exploiting this breakdown in effector-nonhost target interaction, transferring effector target orthologues from non-host to host plants is a strategy to reduce disease.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Effector-triggered Susceptibility ; Host Range ; Oomycete ; Plant Immunity ; Plant–microbe Interactions
Language english
Publication Year 2022
HGF-reported in Year 2022
ISSN (print) / ISBN 0027-8424
e-ISSN 1091-6490
Journal Proceedings of the National Academy of Sciences of the United States of America
Quellenangaben Volume: 119, Issue: 35, Pages: , Article Number: e2114064119 Supplement: ,
Publisher National Academy of Sciences
Reviewing status Peer reviewed
Institute(s) Institute of Network Biology (INET)
POF-Topic(s) 30203 - Molecular Targets and Therapies
Research field(s) Environmental Sciences
PSP Element(s) G-506400-001
Grants
UKRI | Biotechnology and Biological Sciences Research Council (BBSRC)
DOI 10.1073/pnas.2114064119
WOS ID WOS:000883462700003
Scopus ID 85136165701
PubMed ID 35994659
Erfassungsdatum 2022-11-14
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