PuSH - Publication Server of Helmholtz Zentrum München

Export: TXT Text RIS Endnote (RIS) BIB BibTeX
Dantes, Z.* ; Yen, H.Y.* ; Pfarr, N.* ; Winter, C.* ; Steiger, K.* ; Muckenhuber, A.* ; Hennig, A.* ; Lange, S.* ; Engleitner, T.* ; Öllinger, R.* ; Maresch, R.* ; Orben, F.* ; Heid, I.* ; Kaissis, G.* ; Shi, K.* ; Topping, G.* ; Stögbauer, F.* ; Wirth, M.* ; Peschke, K.* ; Papargyriou, A.* ; Rezaee-Oghazi, M.* ; Feldmann, K.* ; Schäfer, A.P.* ; Ranjan, R.* ; Lubeseder-Martellato, C.* ; Stange, D.E.* ; Welsch, T.* ; Martignoni, M.* ; Ceyhan, G.O.* ; Friess, H.* ; Herner, A.* ; Liotta, L.A.* ; Treiber, M.* ; von Figura, G.* ; Abdelhafez, M.* ; Klare, P.* ; Schlag, C.* ; Algül, H.* ; Siveke, J.* ; Braren, R.* ; Weirich, G.* ; Weichert, W.* ; Saur, D.* ; Rad, R.* ; Schmid, R.M.* ; Schneider, G.* ; Reichert, M.*

Implementing cell-free DNA of pancreatic cancer patient-derived organoids for personalized oncology.

JCI insight 5:e137809 (2020)
Publ. Version/Full Text DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
One of the major challenges in using pancreatic cancer patient-derived organoids (PDOs) in precision oncology is the time from biopsy to functional characterization. This is particularly true for endoscopic ultrasound-guided fine-needle aspiration biopsies, typically resulting in specimens with limited tumor cell yield. Here, we tested conditioned media of individual PDOs for cell-free DNA to detect driver mutations already early on during the expansion process to accelerate the genetic characterization of PDOs as well as subsequent functional testing. Importantly, genetic alterations detected in the PDO supernatant, collected as early as 72 hours after biopsy, recapitulate the mutational profile of the primary tumor, indicating suitability of this approach to subject PDOs to drug testing in a reduced time frame. In addition, we demonstrated that this workflow was practicable, even in patients for whom the amount of tumor material was not sufficient for molecular characterization by established means. Together, our findings demonstrate that generating PDOs from very limited biopsy material permits molecular profiling and drug testing. With our approach, this can be achieved in a rapid and feasible fashion with broad implications in clinical practice.
Impact Factor
Scopus SNIP
Altmetric
6.205
1.515
Tags
Annotations
Special Publikation
Hide on homepage

Edit extra information
Edit own tags
Private
Edit own annotation
Private
Hide on publication lists
on hompage
Mark as special
publikation
Publication type Article: Journal article
Document type Scientific Article
Keywords Gastroenterology ; Oncology ; Translation
Language english
Publication Year 2020
HGF-reported in Year 2020
ISSN (print) / ISBN 2379-3708
e-ISSN 2379-3708
Journal JCI insight
Quellenangaben Volume: 5, Issue: 15, Pages: , Article Number: e137809 Supplement: ,
Publisher Clarivate
Publishing Place Ann Arbor, Michigan
Reviewing status Peer reviewed
Institute(s) Helmholtz Artifical Intelligence Cooperation Unit (HAICU)
POF-Topic(s) 30205 - Bioengineering and Digital Health
Research field(s) Enabling and Novel Technologies
PSP Element(s) G-530014-001
DOI 10.1172/jci.insight.137809
PubMed ID 32614802
Erfassungsdatum 2022-09-13
[➜Report correction]