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Transwell migration assay to interrogate human CAR-T cell chemotaxis.
STAR Protoc. 3:101708 (2022)
A major impediment to effective cellular therapies in solid tumors is the limited access of therapeutic cells to the tumor site. One strategy to overcome this challenge is to endow T cells with chemotactic properties required to access tumor tissue. Here, we present a chimeric antigen receptor (CAR)-modified T cell strategy centered around enhanced T cell trafficking. We outline isolation, activation, and transduction of human T cells, as well as techniques for assessing migratory and cytotoxic capacity of CAR-T cells. For complete details on the use and execution of this protocol, please refer to Lesch et al. (2021).
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Biotechnology And Bioengineering ; Cancer ; Cell Isolation ; Cell-based Assays ; Immunology
e-ISSN
2666-1667
Journal
STAR Protocols
Quellenangaben
Volume: 3,
Issue: 4,
Article Number: 101708
Publisher
Elsevier
Publishing Place
Radarweg 29, 1043 Nx Amsterdam, Netherlands
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Unit for Clinical Pharmacology (KKG-EKLiP)
Grants
Hector Foundation
International Doctoral Program i-Target: Immunotargeting of Cancer - Elite Network of Bavaria
Melanoma Research Alliance Grants
Else Kroner-Fresenius-Stiftung
German Cancer Aid
Ernst-Jung-Stiftung
LMU Munich's Institutional Strategy LMUexcellent
Marie-Sklodowska-Curie Program Training Network for Optimizing Adoptive T Cell Therapy of Cancer - H2020 Program of the European Union
Go-Bio-initiative
M4 award - Bavarian Ministry of Economic Affairs
European Research Council
German Research Foundation (DFG)
Fritz-Bender Foundation
Wilhelm-Sander-Stiftung
Deutsche Jose-Carreras Leukamie Stiftung
Bundesministerium fur Bildung und Forschung
International Doctoral Program i-Target: Immunotargeting of Cancer - Elite Network of Bavaria
Melanoma Research Alliance Grants
Else Kroner-Fresenius-Stiftung
German Cancer Aid
Ernst-Jung-Stiftung
LMU Munich's Institutional Strategy LMUexcellent
Marie-Sklodowska-Curie Program Training Network for Optimizing Adoptive T Cell Therapy of Cancer - H2020 Program of the European Union
Go-Bio-initiative
M4 award - Bavarian Ministry of Economic Affairs
European Research Council
German Research Foundation (DFG)
Fritz-Bender Foundation
Wilhelm-Sander-Stiftung
Deutsche Jose-Carreras Leukamie Stiftung
Bundesministerium fur Bildung und Forschung