PuSH - Publication Server of Helmholtz Zentrum München

Zorn, J. ; Schwamberger, S. ; Panzer, W. ; Adler, H. ; Kolb, H.-J.

Transplantation of CD6-depleted peripheral blood stem cells after DLA-haploidentical bone marrow transplantation contributes to engraftment and tolerance in a preclinical model of stem cell transplantation.

Vet. Immunol. Immunopathol. 144, 27-35 (2011)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
Human leukocyte antigen (HLA)-haploidentical stem cell transplantation is an opportunity for nearly all patients lacking an HLA matched stem cell donor. However, graft rejection and graft-versus-host disease (GvHD) as well as infectious complications still result in high treatment-related mortality. Here, we used the dog as a preclinical model for the study of tolerance induction with the aim to optimize and to improve a clinical protocol of haploidentical stem cell transplantation. For this purpose CD6-depleted peripheral blood stem cells (PBSCs) were transfused 6d after transplantation of unmodified bone marrow from dog leukocyte antigen (DLA)-haploidentical littermate donors in order to induce immune tolerance. Besides hematopoietic stem cells CD6-depleted PBSC contain, NK cells and a minority of suppressive CD8-positive cells that may suppress activated T lymphocytes. Recipients were conditioned with, cyclophosphamide and antithymocyte globulin (ATG) preceded by a transfusion of donor buffy coat and either 1, 2 or 3×3.3Gy total body irradiation (TBI). Postgrafting immunosuppression was limited to 30d of cyclosporine and methotrexate. The additional administration of CD6-depleted PBSCs after unmodified marrow could not prevent GvHD, but it may improve engraftment and chimerism after conditioning with 2×3.3Gy TBI. Reasons for incomplete suppression and possible improvements for clinical applications are discussed.
Altmetric
Additional Metrics?
[➜Log in]
Edit extra informations Login
Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Haploidentical stem cell transplantation; CD6-depletion; Dog; Animal model; GvHD; Tolerance
ISSN (print) / ISBN 0165-2427
e-ISSN 1873-2534
Journal Veterinary Immunology and Immunopathology
Quellenangaben Volume: 144, Issue: 1-2, Pages: 27-35 Article Number: , Supplement: ,
Publisher Elsevier
Publishing Place Amsterdam, Netherlands
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) CCG Hematopoetic Cell Transplants (IMI-KHZ)
CF Comparative Medicine (CF-Comparative Medicine)
Research Unit Medical Radiation Physics and Diagnostics (AMSD)