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Open Access Green as soon as Postprint is submitted to ZB.
Long-term single-cell imaging of mammalian stem cells.
Nat. Methods 8, Suppl. S, S30-S35 (2011)
Continuous long-term single-cell observation provides insight into the molecular control of cell fate. This is particularly important for rare and heterogeneous populations of cells, such as mammalian stem cells. The current lack of usable off-the-shelf hardware and software for such experiments makes their implementation technically challenging. Here I discuss the need for continuous single-cell quantification to understand molecular cell fate control as well as organizational and technical solutions for long-term imaging and tracking of stem cells.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
mouse cerebral-cortex; hematopoietic stem; in-vitro; progenitor cells; self-renewal; postnatal astroglia; systems biology; lineage choice; fate; generation
ISSN (print) / ISBN
1548-7091
e-ISSN
1548-7105
Journal
Nature Methods
Quellenangaben
Volume: 8,
Issue: 4,
Pages: S30-S35,
Supplement: Suppl. S
Publisher
Nature Publishing Group
Publishing Place
New York, NY
Reviewing status
Peer reviewed
Institute(s)
Research Unit Stem Cell Dynamics (SCD)