PuSH - Publication Server of Helmholtz Zentrum München

Kaplan, L.* ; Drexler, C.G.* ; Pfaller, A.M.* ; Brenna, S.* ; Wunderlich, K.A.* ; Dimitracopoulos, A.* ; Merl-Pham, J. ; Perez, M.T.* ; Schlötzer-Schrehardt, U.* ; Enzmann, V.* ; Samardzija, M.* ; Puig, B.* ; Fuchs, P.* ; Franze, K.* ; Hauck, S.M. ; Grosche, A.*

Retinal regions shape human and murine Müller cell proteome profile and functionality.

Glia 71, 391-414 (2023)
Publ. Version/Full Text Research data DOI PMC
Open Access Gold (Paid Option)
Creative Commons Lizenzvertrag
The human macula is a highly specialized retinal region with pit-like morphology and rich in cones. How Müller cells, the principal glial cell type in the retina, are adapted to this environment is still poorly understood. We compared proteomic data from cone- and rod-rich retinae from human and mice and identified different expression profiles of cone- and rod-associated Müller cells that converged on pathways representing extracellular matrix and cell adhesion. In particular, epiplakin (EPPK1), which is thought to play a role in intermediate filament organization, was highly expressed in macular Müller cells. Furthermore, EPPK1 knockout in a human Müller cell-derived cell line led to a decrease in traction forces as well as to changes in cell size, shape, and filopodia characteristics. We here identified EPPK1 as a central molecular player in the region-specific architecture of the human retina, which likely enables specific functions under the immense mechanical loads in vivo.
Altmetric
Additional Metrics?
[➜Log in]
Edit extra informations Login
Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Eppk1 ; Müller Cells ; Glial Heterogeneity ; Macula ; Retina; Glial-cells; Epiplakin; Expression; Keratin; Acid; Rna; Identification; Accumulation; Software; Proteins
ISSN (print) / ISBN 0894-1491
e-ISSN 1098-1136
Journal Glia
Quellenangaben Volume: 71, Issue: 2, Pages: 391-414 Article Number: , Supplement: ,
Publisher Wiley
Publishing Place 111 River St, Hoboken 07030-5774, Nj Usa
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) CF Metabolomics & Proteomics (CF-MPC)
Grants Deutsche Forschungsgemeinschaft
ProRetina Foundation Germany
Austrian Science Fund