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Hemme, C.L.* ; Fields, M.W.* ; He, Q.* ; Deng, Y.* ; Lin, L.* ; Tu, Q.* ; Mouttaki, H. ; Zhou, A.* ; Feng, X.* ; Zuo, Z.* ; Ramsay, B.D.* ; He, Z.* ; Wu, L.* ; van Nostrand, J.* ; Xu, J.* ; Tang, Y.J.* ; Wiegel, J.* ; Phelps, T.J.* ; Zhou, J.*

Correlation of genomic and physiological traits of thermoanaerobacter species with biofuel yields.

Appl. Environ. Microbiol. 77, 7998-8008 (2011)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
Thermophilic anaerobic noncellulolytic Thermoanaerobacter species are of great biotechnological importance in cellulosic ethanol production due to their ability to produce high ethanol yields by simultaneous fermentation of hexose and pentose. Understanding the genome structure of these species is critical to improving and implementing these bacteria for possible biotechnological use in consolidated bioprocessing schemes (CBP) for cellulosic ethanol production. Here we describe a comparative genome analysis of two ethanologenic bacteria, Thermoanaerobacter sp. X514 and Thermoanaerobacter pseudethanolicus 39E. Compared to 39E, X514 has several unique key characteristics important to cellulosic biotechnology, including additional alcohol dehydrogenases and xylose transporters, modifications to pentose metabolism, and a complete vitamin B(12) biosynthesis pathway. Experimental results from growth, metabolic flux, and microarray gene expression analyses support genome sequencing-based predictions which help to explain the distinct differences in ethanol production between these strains. The availability of whole-genome sequence and comparative genomic analyses will aid in engineering and optimizing Thermoanaerobacter strains for viable CBP strategies.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords secondary-alcohol dehydrogenase; ethanol-production; clostridium-thermocellum; thermophilic bacterium; shewanella-oneidensis; metabolic pathways; fermentation; cellulose; biomass; strain
ISSN (print) / ISBN 0099-2240
e-ISSN 1098-5336
Journal Applied and Environmental Microbiology
Quellenangaben Volume: 77, Issue: 22, Pages: 7998-8008 Article Number: , Supplement: ,
Publisher American Society for Microbiology (ASM)
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Groundwater Ecology (IGOE)