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Drosophila CENH3 is sufficient for centromere formation.
Science 334, 686-690 (2011)
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CENH3 is a centromere-specific histone H3 variant essential for kinetochore assembly. Despite its central role in centromere function, there has been no conclusive evidence supporting CENH3 as sufficient to determine centromere identity. To address this question, we artificially targeted Drosophila CENH3 (CENP-A/CID) as a CID-GFP-LacI fusion protein to stably integrated lac operator (lacO) arrays. This ectopic CID focus assembles a functional kinetochore and directs incorporation of CID molecules without the LacI-anchor, providing evidence for the self-propagation of the epigenetic mark. CID-GFP-LacI-bound extrachromosomal lacO plasmids can assemble kinetochore proteins and bind microtubules, resulting in their stable transmission for several cell generations even after eliminating CID-GFP-LacI. We conclude that CID is both necessary and sufficient to serve as an epigenetic centromere mark and nucleate heritable centromere function.
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31.377
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
De-novo kinetochore; Chromosome segregation; Budding yeast; Chromatin; Nucleosomes; DNA; Modifiers; Bypasses; Complex
Language
english
Publication Year
2011
HGF-reported in Year
2011
ISSN (print) / ISBN
0036-8075
e-ISSN
1095-9203
Journal
Science
Quellenangaben
Volume: 334,
Issue: 6056,
Pages: 686-690
Publisher
American Association for the Advancement of Science (AAAS)
Reviewing status
Peer reviewed
Institute(s)
CF Monoclonal Antibodies (CF-MAB)
Research Unit Gene Vector (AGV)
Research Unit Gene Vector (AGV)
POF-Topic(s)
30201 - Metabolic Health
30203 - Molecular Targets and Therapies
30203 - Molecular Targets and Therapies
Research field(s)
Helmholtz Diabetes Center
Immune Response and Infection
Immune Response and Infection
PSP Element(s)
G-502210-001
G-501500-001
G-501500-004
G-501500-001
G-501500-004
Scopus ID
80555125093
PubMed ID
22053052
Erfassungsdatum
2011-11-22