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Kukhtevich, I. ; Rivero-Romano, M. ; Rakesh, N. ; Bheda, P. ; Chadha, Y. ; Rosales-Becerra, P.* ; Hamperl, S. ; Bureik, D. ; Dornauer, S. ; Dargemont, C.* ; Kirmizis, A.* ; Schmoller, K.M. ; Schneider, R.

Quantitative RNA imaging in single live cells reveals age-dependent asymmetric inheritance.

Cell Rep. 41:111656 (2022)
Publ. Version/Full Text Research data DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Asymmetric inheritance of cellular content through cell division plays an important role in cell viability and fitness. The dynamics of RNA segregation are so far largely unaddressed. This is partly due to a lack of approaches to follow RNAs over multiple cellular divisions. Here, we establish an approach to quantify RNA dynamics in single cells across several generations in a microfluidics device by tagging RNAs with the diSpinach aptamer. Using S. cerevisiae as a model, we quantitatively characterize intracellular RNA transport from mothers into their buds. Our results suggest that, at cytokinesis, ENO2 diSpinach RNA is preferentially distributed to daughters. This asymmetric RNA segregation depends on the lifespan regulator Sir2 and decreases with increasing replicative age of mothers but does not result from increasing cell size during aging. Overall, our approach opens more opportunities to study RNA dynamics and inheritance in live budding yeast at the single-cell level.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Aging ; Cp: Cell Biology ; Dispinach Aptamer ; Microfluidics ; Rna Aptamer ; Rna Imaging ; Rna Inheritance ; S. Cerevisiae ; Single Cells ; Yeast; Messenger-rna; Saccharomyces-cerevisiae; Damaged Proteins; Life-span; Transcription; Transport; Localization; Segregation; Senescence; Inhibitor
Language english
Publication Year 2022
HGF-reported in Year 2022
ISSN (print) / ISBN 2211-1247
e-ISSN 2211-1247
Journal Cell Reports
Quellenangaben Volume: 41, Issue: 7, Pages: , Article Number: 111656 Supplement: ,
Publisher Cell Press
Publishing Place 50 Hampshire St, Floor 5, Cambridge, Ma 02139 Usa
Reviewing status Peer reviewed
Institute(s) Institute of Functional Epigenetics (IFE)
Institute of Epigenetics and Stem Cells (IES)
POF-Topic(s) 30203 - Molecular Targets and Therapies
30204 - Cell Programming and Repair
Research field(s) Helmholtz Diabetes Center
Stem Cell and Neuroscience
PSP Element(s) G-502800-001
G-554400-001
G-554500-001
Grants Republic of Cyprus through the Research & Innovation Foundation
European Regional Development Fund
French National Research Agency
Human Frontier Science Program
Helmholtz Gesellschaft
DFG
DOI 10.1016/j.celrep.2022.111656
WOS ID 000922746000015
Scopus ID 85142124461
PubMed ID 36384120
Erfassungsdatum 2022-12-07
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