PuSH - Publication Server of Helmholtz Zentrum München: Aqp5<sup>--</sup>/<sup>--</sup> mice exhibit reduced maximal O2 consumption under cold exposure, normal pulmonary gas exchange, and impaired formation of brown adipose tissue.

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Al-Samir, S.* ; Yildirim, A.Ö. ; Sidhaye, V.K.* ; King, L.S.* ; Breves, G.* ; Conlon, T.M. ; Stoeger, C. ; Gailus-Durner, V. ; Fuchs, H. ; Hrabě de Angelis, M. ; Gros, G.* ; Endeward, V.*

Aqp5^--/^-- mice exhibit reduced maximal O2 consumption under cold exposure, normal pulmonary gas exchange, and impaired formation of brown adipose tissue.

Am. J. Physiol.-Regul. Integr. Comp. Physiol. 324, R109-R119 (2022)

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The fundamental body functions that determine maximal O₂ uptake (V_O2,max) have not been studied in Aqp5 ^--/^-- (aquaporin 5, AQP5) mice. We measured V_O2,max to globally assess these functions and then investigated why it was found altered in Aqp5 ^--/^-- mice. V_O2,max was measured by the Helox technique, which elicits maximal metabolic rate by intense cold exposure of the animals. We found V_O2,max reduced in Aqp5 ^--/^-- mice by 20 - 30% compared to WT. Since AQP5 has been implicated to act as a membrane channel for respiratory gases, we studied whether this is due to the known lack of AQP5 in the alveolar epithelial membranes of Aqp5 ^--/^-- mice. Lung function parameters as well as arterial O₂ saturation were normal and identical between Aqp5 ^--/^-- and WT mice, indicating that AQP5 does not contribute to pulmonary O₂ exchange. The cause for the decreased V_O2,max thus might be found in decreased O₂ consumption of an intensely O₂-consuming peripheral organ such as activated BAT. We found indeed that absence of AQP5 greatly reduces the amount of interscapular BAT formed in response to 4 weeks' cold exposure, from 63% in WT to 25% in Aqp5 ^--/^-- animals. We conclude that lack of AQP5 does not affect pulmonary O₂ exchange, but greatly inhibits transformation of white to brown adipose tissue. Since under cold exposure BAT is a major source of the animals' heat production, reduction of BAT likely causes the decrease in V_O2,max under this condition.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Aquaporin 5 ; Alveolar-capillary Barrier ; Gas Channels ; Oxygen Transport Across Membranes ; Pulmonary Diffusion Capacity; Relative Co2/nh3 Selectivities; Oxygen-consumption; Co2 Permeability; Deer Mice; Exercise; Mouse; Aquaporin-1; Peromyscus; Mitochondria; Performance

ISSN (print) / ISBN 0363-6119

e-ISSN 1522-1490

Journal American Journal of Physiology - Regulatory, Integrative and Comparative Physiology

Quellenangaben Volume: 324, Issue: 1, Pages: R109-R119

Publisher American Physiological Society

Publishing Place 6120 Executive Blvd, Suite 600, Rockville, Md, United States

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Institute of Lung Biology (LHI)
Institute of Experimental Genetics (IEG)

Grants German Federal Ministry of Education and Research
Deutsche Forschungsgemeinschaft for financial

PuSH - Publication Server of Helmholtz Zentrum München

Aqp5--/-- mice exhibit reduced maximal O2 consumption under cold exposure, normal pulmonary gas exchange, and impaired formation of brown adipose tissue.

Aqp5^--/^-- mice exhibit reduced maximal O2 consumption under cold exposure, normal pulmonary gas exchange, and impaired formation of brown adipose tissue.