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Abe, K. ; Schauer, T. ; Torres-Padilla, M.E.

Distinct patterns of RNA polymerase II and transcriptional elongation characterize mammalian genome activation.

Cell Rep. 41:111865 (2022)

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	Open Access Gold

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How transcription is regulated as development commences is fundamental to understand how the transcriptionally silent mature gametes are reprogrammed. The embryonic genome is activated for the first time during zygotic genome activation (ZGA). How RNA polymerase II (Pol II) and productive elongation are regulated during this process remains elusive. Here, we generate genome-wide maps of Serine 5 and Serine 2-phosphorylated Pol II during and after ZGA in mouse embryos. We find that both phosphorylated Pol II forms display similar distributions across genes during ZGA, with typical elongation enrichment of Pol II emerging after ZGA. Serine 2-phosphorylated Pol II occurs at genes prior to their activation, suggesting that Serine 2 phosphorylation may prime gene expression. Functional perturbations demonstrate that CDK9 and SPT5 are major ZGA regulators and that SPT5 prevents precocious activation of some genes. Overall, our work sheds molecular insights into transcriptional regulation at the beginning of mammalian development.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Cdk9 ; Cp: Developmental Biology ; Cp: Molecular Biology ; Embryonic Transcription ; Genome Activation ; Mouse Embryo ; Rna Polymerase ; Spt5 ; Transcriptional Elongation ; Zga; Gene-expression; In-vivo; Mouse Oocytes; Muerv-l; Chromatin; Cells; Seq; Spt5; Degradation; Termination

ISSN (print) / ISBN 2211-1247

e-ISSN 2211-1247

Journal Cell Reports

Quellenangaben Volume: 41, Issue: 13, Article Number: 111865

Publisher Cell Press

Publishing Place 50 Hampshire St, Floor 5, Cambridge, Ma 02139 Usa

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Institute of Epigenetics and Stem Cells (IES)

Grants DFG
Helmholtz Association
Uehara Foundation