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Binder, S. ; Rastak, N. ; Karg, E.W. ; Huber, A. ; Kuhn, E. ; Dragan, G.C.* ; Monsé, C.* ; Breuer, D.* ; Di Bucchianico, S. ; Delaval, M.N. ; Oeder, S. ; Sklorz, M. ; Zimmermann, R.

Construction of an in vitro air–liquid interface exposure system to assess the toxicological impact of gas and particle phase of semi-volatile organic compounds.

Toxics 10:730 (2022)

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	Open Access Gold

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Anthropogenic activities and industrialization render continuous human exposure to semi-volatile organic compounds (SVOCs) inevitable. Occupational monitoring and safety implementations consider the inhalation exposure of SVOCs as critically relevant. Due to the inherent properties of SVOCs as gas/particle mixtures, risk assessment strategies should consider particle size-segregated SVOC association and the relevance of released gas phase fractions. We constructed an in vitro air–liquid interface (ALI) exposure system to study the distinct toxic effects of the gas and particle phases of the model SVOC dibutyl phthalate (DBP) in A549 human lung epithelial cells. Cytotoxicity was evaluated and genotoxic effects were measured by the alkaline and enzyme versions of the comet assay. Deposited doses were assessed by model calculations and chemical analysis using liquid chromatography tandem mass spectrometry. The novel ALI exposure system was successfully implemented and revealed the distinct genotoxic effects of the gas and particle phases of DBP. The empirical measurements of cellular deposition and the model calculations of the DBP particle phase were concordant.The model SVOC DBP showed that inferred oxidative DNA damage may be attributed to particle-related effects. While pure gas phase exposure may follow a distinct mechanism of genotoxicity, the contribution of the gas phase to total aerosol was comparably low.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Air–liquid Interface (ali) ; Dibutyl Phthalate (dbp) ; Gas Phase (gp) ; Particle Phase (pp) ; Particle-induced Oxidative Dna Damage ; Semi-volatile Organic Compounds (svocs); Dibutyl Phthalate; Indoor; Svocs; Deposition; Products; Aerosols

ISSN (print) / ISBN 2305-6304

e-ISSN 2305-6304

Journal Toxics

Quellenangaben Volume: 10, Issue: 12, Article Number: 730

Publisher MDPI

Publishing Place St Alban-anlage 66, Ch-4052 Basel, Switzerland

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Cooperation Group Comprehensive Molecular Analytics (CMA)

Grants German Social Accident Insurance (DGUV)