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Collins, A.* ; Møller, P.* ; Gajski, G.* ; Vodenková, S.* ; Abdulwahed, A.* ; Anderson, D.* ; Bankoglu, E.E.* ; Bonassi, S.* ; Boutet-Robinet, E.* ; Brunborg, G.* ; Chao, C.* ; Cooke, M.S.* ; Costa, C.* ; Costa, S.* ; Dhawan, A.* ; de Lapuente, J.* ; Bo', C.D.* ; Dubus, J.* ; Dusinska, M.* ; Duthie, S.J.* ; Yamani, N.E.* ; Engelward, B.* ; Gaivão, I.* ; Giovannelli, L.* ; Godschalk, R.* ; Guilherme, S.* ; Gutzkow, K.B.* ; Habas, K.* ; Hernandez, A.* ; Herrero, O.* ; Isidori, M.* ; Jha, A.N.* ; Knasmüller, S.* ; Kooter, I.M.* ; Koppen, G.* ; Kruszewski, M.* ; Ladeira, C.* ; Laffon, B.* ; Larramendy, M.* ; Hégarat, L.L.* ; Lewies, A.* ; Lewinska, A.* ; Liwszyc, G.E.* ; de Cerain, A.L.* ; Manjanatha, M.* ; Marcos, R.* ; Milić, M.* ; de Andrade, V.M.* ; Moretti, M.* ; Muruzabal, D.* ; Novak, M.* ; Oliveira, R.* ; Olsen, A.K.* ; Owiti, N.* ; Pacheco, M.* ; Pandey, A.K.* ; Pfuhler, S.* ; Pourrut, B.* ; Reisinger, K.* ; Rojas, E.* ; Rundén-Pran, E.* ; Sanz-Serrano, J.* ; Shaposhnikov, S.* ; Sipinen, V.* ; Smeets, K.* ; Stopper, H.* ; Teixeira, J.P.* ; Valdiglesias, V.* ; Valverde, M.* ; van Acker, F.* ; van Schooten, F.J.* ; Vasquez, M.* ; Wentzel, J.F.* ; Wnuk, M.* ; Wouters, A.* ; Žegura, B.* ; Zikmund, T. ; Langie, S.A.S.* ; Azqueta, A.*

Measuring DNA modifications with the comet assay: A compendium of protocols.

Nat. Protoc. 18, 929-989 (2023)
Postprint DOI PMC
Open Access Green
The comet assay is a versatile method to detect nuclear DNA damage in individual eukaryotic cells, from yeast to human. The types of damage detected encompass DNA strand breaks and alkali-labile sites (e.g., apurinic/apyrimidinic sites), alkylated and oxidized nucleobases, DNA-DNA crosslinks, UV-induced cyclobutane pyrimidine dimers and some chemically induced DNA adducts. Depending on the specimen type, there are important modifications to the comet assay protocol to avoid the formation of additional DNA damage during the processing of samples and to ensure sufficient sensitivity to detect differences in damage levels between sample groups. Various applications of the comet assay have been validated by research groups in academia, industry and regulatory agencies, and its strengths are highlighted by the adoption of the comet assay as an in vivo test for genotoxicity in animal organs by the Organisation for Economic Co-operation and Development. The present document includes a series of consensus protocols that describe the application of the comet assay to a wide variety of cell types, species and types of DNA damage, thereby demonstrating its versatility.
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publikation
Publication type Article: Journal article
Document type Review
Language english
Publication Year 2023
HGF-reported in Year 2023
ISSN (print) / ISBN 1754-2189
e-ISSN 1750-2799
Journal Nature Protocols
Quellenangaben Volume: 18, Issue: 3, Pages: 929-989 Article Number: , Supplement: ,
Publisher Nature Publishing Group
Reviewing status Peer reviewed
Institute(s) Institute of Epigenetics and Stem Cells (IES)
POF-Topic(s) 30204 - Cell Programming and Repair
Research field(s) Stem Cell and Neuroscience
PSP Element(s) G-506291-001
DOI 10.1038/s41596-022-00754-y
Scopus ID 85146929563
PubMed ID 36707722
Erfassungsdatum 2023-02-01
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