PuSH - Publication Server of Helmholtz Zentrum München

Export: TXT Text RIS Endnote (RIS) BIB BibTeX
Weiß, M. ; Chanou, A. ; Schauer, T. ; Tvardovskiy, A. ; Meiser, S. ; König, A.-C. ; Schmidt, T. ; Kruse, E. ; Ummethum, H. ; Trauner, M. ; Werner, M. ; Lalonde, M. ; Hauck, S.M. ; Scialdone, A. ; Hamperl, S.

Single-copy locus proteomics of early- and late-firing DNA replication origins identifies a role of Ask1/DASH complex in replication timing control.

Cell Rep. 42:112045 (2023)
Publ. Version/Full Text DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
The chromatin environment at origins of replication is thought to influence DNA replication initiation in eukaryotic genomes. However, it remains unclear how and which chromatin features control the firing of early-efficient (EE) or late-inefficient (LI) origins. Here, we use site-specific recombination and single-locus chromatin isolation to purify EE and LI replication origins in Saccharomyces cerevisiae. Using mass spectrometry, we define the protein composition of native chromatin regions surrounding the EE and LI replication start sites. In addition to known origin interactors, we find the microtubule-binding Ask1/DASH complex as an origin-regulating factor. Strikingly, tethering of Ask1 to individual origin sites advances replication timing (RT) of the targeted chromosomal domain. Targeted degradation of Ask1 globally changes RT of a subset of origins, which can be reproduced by inhibiting microtubule dynamics. Thus, our findings mechanistically connect RT and chromosomal organization via Ask1/DASH with the microtubule cytoskeleton.
Impact Factor
Scopus SNIP
Web of Science
Times Cited
Altmetric
8.800
0.000
1
Tags
Annotations
Special Publikation
Hide on homepage

Edit extra information
Edit own tags
Private
Edit own annotation
Private
Hide on publication lists
on hompage
Mark as special
publikation
Publication type Article: Journal article
Document type Scientific Article
Keywords Ask1 Protein ; Cp: Molecular Biology ; Dash Complex ; Dna Replication Origin ; Affinity Purification ; Locus-specific Chromatin Isolation ; Microtubule Cytoskeleton ; Origin Chromatin Structure ; Replication Efficiency ; Replication Timing ; Site-specific Recombination; Kinetochore-microtubule Interface; Yeast; Chromatin; Binding; Recognition; Activation; Initiation; Sequence; Dynamics; Time
Language english
Publication Year 2023
HGF-reported in Year 2023
ISSN (print) / ISBN 2211-1247
e-ISSN 2211-1247
Journal Cell Reports
Quellenangaben Volume: 42, Issue: 2, Pages: , Article Number: 112045 Supplement: ,
Publisher Cell Press
Publishing Place 50 Hampshire St, Floor 5, Cambridge, Ma 02139 Usa
Reviewing status Peer reviewed
Institute(s) Institute of Epigenetics and Stem Cells (IES)
Institute of Functional Epigenetics (IFE)
CF Metabolomics & Proteomics (CF-MPC)
Institute of Computational Biology (ICB)
POF-Topic(s) 30204 - Cell Programming and Repair
30203 - Molecular Targets and Therapies
30205 - Bioengineering and Digital Health
Research field(s) Stem Cell and Neuroscience
Helmholtz Diabetes Center
Enabling and Novel Technologies
PSP Element(s) G-554500-001
G-506200-001
G-502800-001
G-506290-001
G-505700-001
G-502899-701
G-503800-001
A-630700-001
Grants
Helmholtz Gesellschaft
European Research Council (ERC starting grant)
DFG
DOI 10.1016/j.celrep.2023.112045
WOS ID 000961147700001
Scopus ID 85146843974
PubMed ID 36701236
Erfassungsdatum 2023-02-01
[➜Report correction]