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Harris, C.* ; Scheibe, M.* ; Wongpalee, S.P.* ; Liu, W.* ; Cornett, E.M.* ; Vaughan, R.* ; Li, X.* ; Chen, W.* ; Xue, Y.* ; Zhong, Z.* ; Yen, L.* ; Barshop, W.D.* ; Rayatpisheh, S.* ; Gallego-Bartolome, J.* ; Groth, M. ; Wang, Z.* ; Wohlschlegel, J.A.* ; Du, J.* ; Rothbart, S.B.* ; Butter, F.* ; Jacobsen, S.E.*

A DNA methylation reader complex that enhances gene transcription.

Science 362, 1182-1186 (2018)
Postprint DOI PMC
Open Access Green
DNA methylation generally functions as a repressive transcriptional signal, but it is also known to activate gene expression. In either case, the downstream factors remain largely unknown. By using comparative interactomics, we isolated proteins in Arabidopsis thaliana that associate with methylated DNA. Two SU(VAR)3-9 homologs, the transcriptional antisilencing factor SUVH1, and SUVH3, were among the methyl reader candidates. SUVH1 and SUVH3 bound methylated DNA in vitro, were associated with euchromatic methylation in vivo, and formed a complex with two DNAJ domain-containing homologs, DNAJ1 and DNAJ2. Ectopic recruitment of DNAJ1 enhanced gene transcription in plants, yeast, and mammals. Thus, the SUVH proteins bind to methylated DNA and recruit the DNAJ proteins to enhance proximal gene expression, thereby counteracting the repressive effects of transposon insertion near genes.
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Publication type Article: Journal article
Document type Scientific Article
Language english
Publication Year 2018
HGF-reported in Year 2018
ISSN (print) / ISBN 0036-8075
e-ISSN 1095-9203
Journal Science
Quellenangaben Volume: 362, Issue: 6419, Pages: 1182-1186 Article Number: , Supplement: ,
Publisher American Association for the Advancement of Science (AAAS)
Reviewing status Peer reviewed
Institute(s) Institute of Biochemical Plant Pathology (BIOP)
POF-Topic(s) 30202 - Environmental Health
Research field(s) Environmental Sciences
PSP Element(s) G-504900-001
Grants Howard Hughes Medical Institute
NIGMS NIH HHS
DOI 10.1126/science.aar7854
PubMed ID 30523112
Erfassungsdatum 2023-03-01
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