Open Access Green as soon as Postprint is submitted to ZB.
Genetically encoded barcodes for correlative volume electron microscopy.
Nat. Biotechnol. 41, 1734–1745 (2023)
While genetically encoded reporters are common for fluorescence microscopy, equivalent multiplexable gene reporters for electron microscopy (EM) are still scarce. Here, by installing a variable number of fixation-stable metal-interacting moieties in the lumen of encapsulin nanocompartments of different sizes, we developed a suite of spherically symmetric and concentric barcodes (EMcapsulins) that are readable by standard EM techniques. Six classes of EMcapsulins could be automatically segmented and differentiated. The coding capacity was further increased by arranging several EMcapsulins into distinct patterns via a set of rigid spacers of variable length. Fluorescent EMcapsulins were expressed to monitor subcellular structures in light and EM. Neuronal expression in Drosophila and mouse brains enabled the automatic identification of genetically defined cells in EM. EMcapsulins are compatible with transmission EM, scanning EM and focused ion beam scanning EM. The expandable palette of genetically controlled EM-readable barcodes can augment anatomical EM images with multiplexed gene expression maps.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Metallothionein; Protein; Tag; Binding; Pb(ii); Cells
ISSN (print) / ISBN
1087-0156
e-ISSN
1546-1696
Journal
Nature Biotechnology
Quellenangaben
Volume: 41,
Pages: 1734–1745
Publisher
Nature Publishing Group
Publishing Place
New York, NY
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Insitute of Synthetic Biomedicine (ISBM)
Research Unit Analytical Pathology (AAP)
Helmholtz Artifical Intelligence Cooperation Unit (HAICU)
Research Unit Analytical Pathology (AAP)
Helmholtz Artifical Intelligence Cooperation Unit (HAICU)