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Wang, Q.* ; Hoene, M.* ; Hu, C.* ; Fritsche, L. ; Ahrends, R.* ; Liebisch, G.* ; Ekroos, K.* ; Fritsche, A. ; Birkenfeld, A.L. ; Liu, X.* ; Zhao, X.* ; Li, Q.* ; Su, B.* ; Peter, A. ; Xu, G.* ; Lehmann, R.

Ex vivo instability of lipids in whole blood: Preanalytical recommendations for clinical lipidomics studies.

J. Lipid Res. 64:100378 (2023)

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Reliability, robustness, and interlaboratory comparability of quantitative measurements is critical for clinical lipidomics studies. Lipids' different ex vivo stability in blood bears the risk of misinterpretation of data. Clear recommendations for the process of blood sample collection are required. We studied by UHPLC-high resolution mass spectrometry, as part of the "Preanalytics interest group" of the International Lipidomics Society, the stability of 417 lipid species in EDTA whole blood after exposure to either 4°C, 21°C, or 30°C at six different time points (0.5 h-24 h) to cover common daily routine conditions in clinical settings. In total, >800 samples were analyzed. 325 and 288 robust lipid species resisted 24 h exposure of EDTA whole blood to 21°C or 30°C, respectively. Most significant instabilities were detected for FA, LPE, and LPC. Based on our data, we recommend cooling whole blood at once and permanent. Plasma should be separated within 4 h, unless the focus is solely on robust lipids. Lists are provided to check the ex vivo (in)stability of distinct lipids and potential biomarkers of interest in whole blood. To conclude, our results contribute to the international efforts towards reliable and comparable clinical lipidomics data paving the way to the proper diagnostic application of distinct lipid patterns or lipid profiles in the future.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Clinical Lipidomics ; Blood ; Lipid Stability ; Preanalytical ; Sample Collection; Human Plasma; Metabolomics; Risk

ISSN (print) / ISBN 0022-2275

e-ISSN 1539-7262

Journal Journal of Lipid Research

Quellenangaben Volume: 64, Issue: 6, Article Number: 100378

Publisher American Society for Biochemistry and Molecular Biology

Publishing Place Radarweg 29, 1043 Nx Amsterdam, Netherlands

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Institute of Diabetes Research and Metabolic Diseases (IDM)

Grants
German Federal Ministry of Education and Research (BMBF)
Innovation program
DICP
Youth Innovation Promotion Association CAS
Chinese Academy of Sciences (CAS)-President 's International Fellowship Initiative
DFG/NSFC Sino-German mobility program
National Natural Science Foundation of China