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Fischer, F. ; Doll, A.* ; Uereyener, D. ; Roenneberg, S.* ; Hillig, C. ; Weber, L.* ; Hackert, V.* ; Meinel, M. ; Farnoud, A. ; Seiringer, P.* ; Thomas, J. ; Anand, P.* ; Graner, L.* ; Schlenker, F.* ; Zengerle, R.* ; Jonsson, P.* ; Jargosch, M. ; Theis, F.J. ; Schmidt-Weber, C.B. ; Biedermann, T.* ; Howell, M.* ; Reich, K.* ; Eyerich, K.* ; Menden, M.P. ; Garzorz-Stark, N.* ; Lauffer, F.* ; Eyerich, S.

Gene expression-based molecular test as diagnostic aid for the differential diagnosis of psoriasis and eczema in formalin-fixed and paraffin-embedded tissue, microbiopsies, and tape strips.

J. Invest. Dermatol. 143, 1461-1469.e5 (2023)
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Highly effective targeted therapies are available to treat noncommunicable chronic inflammatory skin diseases. In contrast, the exact diagnosis of noncommunicable chronic inflammatory skin diseases is complicated by its complex pathogenesis and clinical and histological overlap. Particularly, the differential diagnosis of psoriasis and eczema can be challenging in some cases, and molecular diagnostic tools need to be developed to support a gold standard diagnosis. The aim of this work was to develop a real-time PCR-based molecular classifier to distinguish psoriasis from eczema in formalin-fixed and paraffin-embedded-fixed skin samples and to evaluate the use of minimally invasive microbiopsies and tape strips for molecular diagnosis. In this study, we present a formalin-fixed and paraffin-embedded-based molecular classifier that determines the probability for psoriasis with a sensitivity/specificity of 92%/100%, respectively, and an area under the curve of 0.97, delivering comparable results to our previous published RNAprotect-based molecular classifier. The psoriasis probability, as well as levels of NOS2 expression, positively correlated with the disease hallmarks of psoriasis and negatively with eczema hallmarks. Furthermore, minimally invasive tape strips and microbiopsies were effectively used to differentiate psoriasis from eczema. In summary, the molecular classifier offers broad usage in pathology laboratories as well as outpatient settings and can support the differential diagnosis of noncommunicable chronic inflammatory skin diseases on a molecular level using formalin-fixed and paraffin-embedded tissue, microbiopsies, and tape strips.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Dermatitis; Melanoma
Language english
Publication Year 2023
HGF-reported in Year 2023
ISSN (print) / ISBN 0022-202X
e-ISSN 1523-1747
Journal Journal of Investigative Dermatology, The
Quellenangaben Volume: 143, Issue: 8, Pages: 1461-1469.e5 Article Number: , Supplement: ,
Publisher Elsevier
Publishing Place New York, NY
Reviewing status Peer reviewed
Institute(s) Institute for Allergy Research (IAF)
Institute of Computational Biology (ICB)
POF-Topic(s) 30202 - Environmental Health
30205 - Bioengineering and Digital Health
Research field(s) Allergy
Enabling and Novel Technologies
PSP Element(s) G-505490-001
G-503800-001
G-554700-001
G-505400-001
Grants Munich School for Data Science
IGSSE graduate school
Medical Valley Award
DOI 10.1016/j.jid.2023.02.015
WOS ID 001044726500001
Scopus ID 85151536276
PubMed ID 36889660
Erfassungsdatum 2023-10-06
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