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Sikkenk, D.J.* ; Sterkenburg, A.J.* ; Schmidt, I.* ; Gorpas, D. ; Nagengast, W.B.* ; Consten, E.C.J.*

Detection of tumour-targeted IRDye800CW tracer with commercially available laparoscopic surgical systems.

Diagnostics 13:21 (2023)
Publ. Version/Full Text DOI PMC
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(1) Introduction: Near-infrared fluorescence (NIRF) combined with tumour-targeted tracers, such as bevacizumab-800CW, could aid surgical decision-making. This study explored the use of IRDye800CW, conjugated to bevacizumab, with four commercially available NIRF laparoscopes optimised for indocyanine green (ICG). (2) Methods: A (lymph node) phantom was made from a calibration device for NIRF and tissue-mimicking material. Serial dilutions of bevacizumab-800CW were made and ICG functioned as a reference. System settings, working distance, and thickness of tissue-mimicking material were varied to assess visibility of the fluorescence signal and tissue penetration. Tests were performed with four laparoscopes: VISERA ELITE II, Olympus; IMAGE1 S™ 4U Rubina, KARL STORZ; ENDOCAM Logic 4K platform, Richard Wolf; da Vinci Xi, Intuitive Surgical. (3) Results: The lowest visible bevacizumab-800CW concentration ranged between 13-850 nM (8-512 times diluted stock solution) for all laparoscopes, but the tracer was not visible through 0.8 cm of tissue in all systems. In contrast, ICG was still visible at a concentration of 0.4 nM (16,384 times diluted) and through 1.6-2.4 cm of tissue. Visibility and tissue penetration generally improved with a reduced working distance and manually adjusted system settings. (4) Conclusion: Depending on the application, bevacizumab-800CW might be sufficiently visible with current laparoscopes, but optimisation would widen applicability of tumour-targeted IRDye800CW tracers.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Irdye800cw ; Fluorescence Guided Surgery ; Fluorescence Molecular Imaging ; Image-guided Surgery ; Indocyanine Green (icg) ; Near-infrared Fluorescence (nirf) ; Targeted Tracer; Fluorescence-guided Surgery; Indocyanine-green; Thermal-stability; Photostability; Safety; Light
ISSN (print) / ISBN 2075-4418
e-ISSN 2075-4418
Journal Diagnostics
Quellenangaben Volume: 13, Issue: 9, Pages: , Article Number: 21 Supplement: ,
Publisher MDPI
Publishing Place St Alban-anlage 66, Ch-4052 Basel, Switzerland
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Biological and Medical Imaging (IBMI)